Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080657914> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2080657914 endingPage "334" @default.
- W2080657914 startingPage "323" @default.
- W2080657914 abstract "Phosphofructokinase-1 from Saccharomyces cerevisiae is an octameric enzyme comprising two non-identical subunits, α and β, which are encoded by the unlinked genes PFK1 and PFK2. In this paper, assembly and reactivation of the enzyme have been studied in cell-free extracts of single-deletion mutants. In contrast to the previously described lack of phosphofructokinase-1 activity in cell-free extracts of these mutants, we could measure a temporary enzyme activity immediately after lysis of protoplasts. This result supports the assumption that each of the subunits forms an enzyme structure which is active in vivo but not stable after cell disruption. Upon mixing of separately prepared cell-free extracts of both deletion mutants very low activity could be measured. About 40% of the wild-type activity was regained when both mutants were mixed prior to disruption. The reactivation rate could be slightly increased by addition of ATP and fructose 6-phosphate and was found to be a function of the growth state, particularly of the β-subunit-carrying cells. The individual subunits did not interact with Cibacron Blue F3G-A, a biomimetic ligand of phosphofructokinase-1. After reassembly of both subunits in vitro a strong affinity of the reconstituted phosphofructokinase-1 to the dye-ligand was observed. The inability of the subunits to reconstitute under certain conditions seems to result from alterations of the intracellular environment following disruption. These changes give rise to induce an unproductive side reaction like self-aggregation of the subunits. Because reconstitution of phosphofructokinase-1 from S. cerevisiae behaves in a similar way to that of hemoglobin and luciferase, we would speculate a general mechanism for assembly of oligomeric proteins in vivo. © 1998 John Wiley & Sons, Ltd." @default.
- W2080657914 created "2016-06-24" @default.
- W2080657914 creator A5006336721 @default.
- W2080657914 creator A5036339128 @default.
- W2080657914 creator A5060480453 @default.
- W2080657914 creator A5069577467 @default.
- W2080657914 date "1998-03-15" @default.
- W2080657914 modified "2023-10-17" @default.
- W2080657914 title "Assembly of phosphofructokinase-1 fromSaccharomyces cerevisiae in extracts of single-deletion mutants" @default.
- W2080657914 cites W1547762530 @default.
- W2080657914 cites W1638961546 @default.
- W2080657914 cites W1846628205 @default.
- W2080657914 cites W1859876210 @default.
- W2080657914 cites W1969022698 @default.
- W2080657914 cites W1969516714 @default.
- W2080657914 cites W1972719789 @default.
- W2080657914 cites W1997470132 @default.
- W2080657914 cites W2004390444 @default.
- W2080657914 cites W2020750285 @default.
- W2080657914 cites W2033411840 @default.
- W2080657914 cites W2034689111 @default.
- W2080657914 cites W2048487254 @default.
- W2080657914 cites W2057755265 @default.
- W2080657914 cites W2060163401 @default.
- W2080657914 cites W2075497250 @default.
- W2080657914 cites W2076415952 @default.
- W2080657914 cites W2076659213 @default.
- W2080657914 cites W2125474289 @default.
- W2080657914 cites W2414137315 @default.
- W2080657914 cites W4293247451 @default.
- W2080657914 doi "https://doi.org/10.1002/(sici)1097-0061(19980315)14:4<323::aid-yea223>3.0.co;2-w" @default.
- W2080657914 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9559541" @default.
- W2080657914 hasPublicationYear "1998" @default.
- W2080657914 type Work @default.
- W2080657914 sameAs 2080657914 @default.
- W2080657914 citedByCount "20" @default.
- W2080657914 countsByYear W20806579142014 @default.
- W2080657914 countsByYear W20806579142017 @default.
- W2080657914 countsByYear W20806579142018 @default.
- W2080657914 crossrefType "journal-article" @default.
- W2080657914 hasAuthorship W2080657914A5006336721 @default.
- W2080657914 hasAuthorship W2080657914A5036339128 @default.
- W2080657914 hasAuthorship W2080657914A5060480453 @default.
- W2080657914 hasAuthorship W2080657914A5069577467 @default.
- W2080657914 hasConcept C104292427 @default.
- W2080657914 hasConcept C104317684 @default.
- W2080657914 hasConcept C122245378 @default.
- W2080657914 hasConcept C143065580 @default.
- W2080657914 hasConcept C181199279 @default.
- W2080657914 hasConcept C20251656 @default.
- W2080657914 hasConcept C2777576037 @default.
- W2080657914 hasConcept C2779222958 @default.
- W2080657914 hasConcept C2779372652 @default.
- W2080657914 hasConcept C55493867 @default.
- W2080657914 hasConcept C79879829 @default.
- W2080657914 hasConcept C86803240 @default.
- W2080657914 hasConcept C95444343 @default.
- W2080657914 hasConceptScore W2080657914C104292427 @default.
- W2080657914 hasConceptScore W2080657914C104317684 @default.
- W2080657914 hasConceptScore W2080657914C122245378 @default.
- W2080657914 hasConceptScore W2080657914C143065580 @default.
- W2080657914 hasConceptScore W2080657914C181199279 @default.
- W2080657914 hasConceptScore W2080657914C20251656 @default.
- W2080657914 hasConceptScore W2080657914C2777576037 @default.
- W2080657914 hasConceptScore W2080657914C2779222958 @default.
- W2080657914 hasConceptScore W2080657914C2779372652 @default.
- W2080657914 hasConceptScore W2080657914C55493867 @default.
- W2080657914 hasConceptScore W2080657914C79879829 @default.
- W2080657914 hasConceptScore W2080657914C86803240 @default.
- W2080657914 hasConceptScore W2080657914C95444343 @default.
- W2080657914 hasIssue "4" @default.
- W2080657914 hasLocation W20806579141 @default.
- W2080657914 hasLocation W20806579142 @default.
- W2080657914 hasOpenAccess W2080657914 @default.
- W2080657914 hasPrimaryLocation W20806579141 @default.
- W2080657914 hasRelatedWork W1572277225 @default.
- W2080657914 hasRelatedWork W1989245391 @default.
- W2080657914 hasRelatedWork W2016785529 @default.
- W2080657914 hasRelatedWork W2041752811 @default.
- W2080657914 hasRelatedWork W2060959141 @default.
- W2080657914 hasRelatedWork W2086285855 @default.
- W2080657914 hasRelatedWork W2089980899 @default.
- W2080657914 hasRelatedWork W2093076661 @default.
- W2080657914 hasRelatedWork W2111858864 @default.
- W2080657914 hasRelatedWork W2135758139 @default.
- W2080657914 hasVolume "14" @default.
- W2080657914 isParatext "false" @default.
- W2080657914 isRetracted "false" @default.
- W2080657914 magId "2080657914" @default.
- W2080657914 workType "article" @default.