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• W2080681517 abstract "This study compared the performance of a flow cytometric immunoassay for antineutrophil cytoplasmic antibodies (ANCA) directed against proteinase 3 (PR3) and myeloperoxidase (MPO), with indirect immunofluorescence (IIF) and ELISAs from 12 different manufacturers. Sera were from patients with active (n = 55) or treated (n = 68) small vessel vasculitis, or inflammatory bowel disease (IBD, n = 22). The immunoassay specificity was 88% compared with 96% for IIF and 94% (median, range 91–96%) for both ELISAs. Its sensitivity in treated disease was 82% compared with 84% for IIF and 69% (median, range 57–82%) for the ELISAs. The immunoassay's specificity was 88% which was the same as the median for both ELISAs (range 84–95%). The PR3- and MPO-ANCA immunoassay was almost as sensitive as IIF, and more sensitive than, but just as specific as, most ELISAs, in detecting ANCA in active and treated vasculitis. A major advantage of this assay is its ability to be further modified to simultaneously screen for a panel of autoantibodies relevant to vasculitis." @default.
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• W2080681517 date "2008-07-01" @default.
• W2080681517 modified "2023-09-23" @default.
• W2080681517 title "Evaluation of a multiplex flow cytometric immunoassay to detect PR3- and MPO-ANCA in active and treated vasculitis, and in inflammatory bowel disease (IBD)" @default.
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• W2080681517 doi "https://doi.org/10.1016/j.jim.2008.03.012" @default.
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