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• W2080700947 abstract "Avenaciolide produces an initial stimulation of mitochondrial respiration followed at high doses of the drug by a decline in respiration to less than the unstimulated rate; under these conditions the mitochondria are insensitive to ADP and to uncoupler. At lower avenaciolide concentrations followed by ADP there is a sustained acceleration of respiration which is sensitive to EDTA and oligomycin, pointing to the existence of a Mg-requiring ATPase. Spectrophotometric tests with bromthymol blue and fluorimetry show a similarity between the responses to avenaciolide and divalent cations. Mitochondrial contents of substrate anions and cations are altered by avenaciolide; the extent of the changes depend on the level of the drug used and also on the composition of the medium. If K+ is present with an energy source, the uptake of K+ at the start of an incubation is enhanced by avenaciolide when supplied at less than 25–30 nmole/mg protein, and the K+ gain is accompanied by an uptake of substrate anion; at higher concentrations of avenaciolide the direction of flow is reversed with loss of K+, divalent cations, and substrate anions. In potassium-free media, or in the absence of energy only losses of ions are found. Addition of avenaciolide to mitochondria onto which [14C]octyl malonate had previously been adsorbed results in a discharge of the labeled compound." @default.
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• W2080700947 date "1974-06-01" @default.
• W2080700947 modified "2023-10-17" @default.
• W2080700947 title "Ionophoric actions of avenaciolide on mitochondrial cations" @default.
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• W2080700947 doi "https://doi.org/10.1016/0003-9861(74)90201-x" @default.
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