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• W2080706177 abstract "We constructed a cDNA library with mRNA pooled from 4- to 6-day-old Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) seedlings. To identify genes with increased expression during germination and early seedling development, the library was screened differentially with cDNA probes synthesized using mRNA isolated from mature seeds and 6-day-old seedlings. Partial DNA sequence analysis and predicted amino acid sequences revealed cDNA clones with homology to several plant proteins including: a chaperonin 60β (cpn60β), a low molecular weight heat shock protein (LMW HSP), a luminal binding protein (BiP), a type II chlorophyll a/b-binding protein (CAB) and a cysteine protease. In mature seeds, transcripts corresponding to each cDNA clone were detected and their levels increased after 2 days of exposure to the germination conditions. The clone that shares identity with the cysteine protease (DF3-3) and DF6-7 had peak transcript levels during the germination phase, whereas the cpn60β (DF3), a LMW HSP (DF4-5), BiP (DF5-1) and CAB (DF6-3) showed peaks during the post-germination phase. All the transcripts were present at lower levels in 10-week-old needles except for CAB. As a comparison, an additional clone which was present at similar levels in both mature seeds and in 6-day-old seedlings was isolated and found to be homologous to a NADPH-cytochrome P450 reductase (CPR) (EC 1.6.2.4). The expression of CPR changed little during germination and early seedling development with lower transcript levels in 10-week-old needles. The significance of the individual genes, their expression, and possible roles during germination is discussed." @default.
• W2080706177 created "2016-06-24" @default.
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• W2080706177 date "1995-03-01" @default.
• W2080706177 modified "2023-10-18" @default.
• W2080706177 title "The molecular characterization of a set of cDNAs differentially expressed during Douglas-fir germination and early seedling development" @default.
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• W2080706177 doi "https://doi.org/10.1111/j.1399-3054.1995.tb00863.x" @default.
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