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- W2080719993 abstract "One focus of our research is to investigate the helix-coil transition of unusual DNA structures by comparing optical and calorimetric melting curves with fluorescence melts obtained by observing the fluorescence changes of 2-aminopurine (AP) when incorporated into DNA. In this work, we used a combination of temperature-dependent UV, circular dichroism (CD) and fluorescence spectroscopies, and differential scanning calorimetric (DSC) techniques to investigate the unfolding of: a) an intramolecular pyrimidine triplex, A3APA3C5T7C5T7; b) a G-quadruplex, G2T2G2TAPTG2T2G2; and c) a dodecamer duplex, CGCGAAPTTCGCG. Our experimental observations are as follows. a) All melting curves of the triplex show similar monophasic transitions with TMs of 31 °C, ΔHvHs of 38 kcal/mol and ΔHDSC of 70 kcal/mol. Thus, this triplex unfolds in a non-two state fashion similar to the unfolding of the unmodified triplex but with a lower endothermic heat (by 12 kcal/mol). This indicates the presence of AP contributes with lower stacking interactions. b) The G-quadruplex also unfolds in similar monophasic transitions: TMs of 52°C, ΔHvHs of 44 kcal/mol and ΔHDSC of 36 kcal/mol. The unstacking of AP follows the overall cooperative unfolding of the whole molecule. This quadruplex unfolds in two-state fashion similar to the unfolding of the unmodified quadruplex but with a higher endothermic heat (by 14 kcal/mol). This indicates that the presence of AP induces higher stacking contributions. c) All melting curves of the dodecamer duplex show biphasic transitions. The TM of the first transition depends on strand concentration while the TM of the second one is independent, consistent with the presence of a duplex ←→ hairpin ←→ random coil equilibria. The central placement of AP destabilizes the duplex state, while does not affect the hairpin state. Supported by Grant MCB-0616005 from NSF." @default.
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- W2080719993 date "2009-02-01" @default.
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- W2080719993 title "Helix-Coil Transitions of Unusual DNA Structures by Measuring the Fluorescence Changes of 2-Aminopurine When Incorporated into DNA" @default.
- W2080719993 doi "https://doi.org/10.1016/j.bpj.2008.12.1741" @default.
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