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• W2080725093 abstract "We describe the construction of restriction and genetic maps of plasmid pMOL28, which has a size of approximately 180 kb. To do so, partialBamHI-digested DNA of pMOL28 was cloned into cosmid pLAFR3, which can package up to 20–30 kb inserted DNA. Subsequently, a cosmid walking strategy, combined withBamHI orEcoRI restriction analysis and hybridization, was used to construct the restriction maps for both enzymes. On these maps, 35BamHI fragments and 29EcoRI fragments were placed, accounting for a total size of approximately 180 kb. We also analyzed several rearranged derivatives of pMOL28 that were obtained after a process of temperature-induced mortality and mutagenesis (TIMM), which is characteristic forAlcaligenes eutrophusCH34 and related strains. The restriction and genetic maps of pMOL50 (222 kb), an enlarged derivative of pMOL28 obtained after TIMM, were constructed. By comparing the pMOL28 and pMOL50 maps, at least two transposable elements were identified which participated in the formation of pMOL50 from pMOL28 during TIMM. These transposable elements were IS1086,which was recently sequenced, and a new element named IS1089,which is located on the 44-kb inserted DNA fragment in pMOL50. Partial sequencing of IS1089revealed similarity of this element with IS1071of the chlorobenzoate catabolic transposon Tn5271ofAlcaligenessp. BR60." @default.
• W2080725093 created "2016-06-24" @default.
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• W2080725093 date "1997-01-01" @default.
• W2080725093 modified "2023-09-27" @default.
• W2080725093 title "Genetic and Physical Maps of theAlcaligenes eutrophusCH34 Megaplasmid pMOL28 and Its Derivative pMOL50 Obtained after Temperature-Induced Mutagenesis and Mortality" @default.
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• W2080725093 doi "https://doi.org/10.1006/plas.1996.1274" @default.
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