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• W2080756164 abstract "A simple, rapid, sensitive and selective liquid chromatography–tandem mass spectrometry method was developed and validated for the quantification of azithromycin in rabbit conjunctiva tissues using roxithromycin as internal standard. Following a deproteinization procedure, the samples were eluted isocratically at a flow rate of 0.3 mL/min utilizing a mobile phase containing of 10 mM ammonium acetate (adjusted pH to 5.2 with 0.1% acetic acid)–methanol (18:82, v/v) and a SHISEIDO CAPCELL PAK C18 (3.0 mm × 75 mm, 3 μm). Azithromycin and its internal standard were measured by a triple-quadrupole mass spectrometer in the selected reaction monitoring (SRM) mode with precursor-to-product qualifier transition m/z 375 [M+2H]2+ → 591 and m/z 837 [M+H]+ → 679 respectively. The method demonstrated that good linearity ranged from 10 to 2000 ng/mL with r = 0.9998. The lower limit of quantification for azithromycin in conjunctiva tissues was 10 ng/mL with good accuracy and precision. The intra- and inter-day precision (RSD) values were below 15% and accuracy (%) ranged from 90% to 110% at all QC levels. The method was applicable to ocular pharmacokinetic studies of azithromycin." @default.
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• W2080756164 date "2010-05-01" @default.
• W2080756164 modified "2023-10-03" @default.
• W2080756164 title "Rapid, sensitive and selective liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the quantification of topically applied azithromycin in rabbit conjunctiva tissues" @default.
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• W2080756164 doi "https://doi.org/10.1016/j.jpba.2009.12.001" @default.
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