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• W2080758957 abstract "Abstract The photo-sensitised oxidation of phenyl linolenate and phenyl arachidonate was studied using four different types of dye sensitiser (methylene blue (MB), erythrosin (ER), haematoporphyrin (HP) and riboflavin (RF)). The primary product formation was analysed by UV-visible spectroscopy, peroxide value (PV) determinations and a combination of chromatographic techniques. The initially formed monohydroperoxides (MHP) were catalytically hydrogenated to their saturated hydroxy derivatives and then separated and quantified by normal phase high performance liquid chromatography (HPLC) for the determination of the positional isomer distribution. The separated fractions were trans-esterified to their methyl esters and the identity of the resulting 9,10,12,13,15 and 16-hydroxystearates and the 5,6,8,9,11,12,14 and 15-hydroxyarachidates (cicosanoates) confirmed by gas chromatography-mass spectroscopy analysis (GCMS). In addition, the identity of the 9,10,12 and 13-hydroxystearates were confirmed by specific synthesis. The balance between free radical (Type I) and singlet oxygen (Type II) mechanisms of photo-oxidation was determined from the relative proportions of the positional isomers. The radical contribution increased with the degree of unsaturation of the substrate and showed small variations with the nature of the sensitiser." @default.
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• W2080758957 date "1992-09-01" @default.
• W2080758957 modified "2023-09-25" @default.
• W2080758957 title "Synthesis and characterisation of the photosensitised oxidation reaction products of phenyl esters of linolenic and arachidonic acids" @default.
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• W2080758957 doi "https://doi.org/10.1016/0009-3084(92)90094-6" @default.
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