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• W2080764575 abstract "A novel nano Fe <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>3</sub> O <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>4</sub> -Au modified amperometric immunosensor for rapid separation-free determination of human immunodeficiency virus (HIV) p24 antigen in acquired immunodeficiency syndrome (AEDs) patients' serum is proposed. Firstly, one novel sebacato-bridged Cu(II) phen complex Cu(phen)L <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2</sub> (referred to as CuL, H <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2</sub> L=sebacic acid; phen=1,10phenanthroline) was absorbed on the surface of glassy carbon electrode (GCE), then gold magnetic nanoparticles Fe <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>3</sub> O <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>4</sub> Au/Chit composite was immobilized onto the surface of CuL modified GCE by its excellent membrane-forming ability, and finally the anti-p24 was adsorbed onto the surface of Fe <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>3</sub> O <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>4</sub> -Au colloid to construct an GCE|CuL/Fe <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>3</sub> O <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>4</sub> -Au/Ab-p24 immunosensor. The modified electrode were characterized by cyclic voltammetry (CV), scanning electron microscope (SEM), and X-ray photo electron spectrum (XPS). Based on a competition immunoreaction, CV method for p24 antigen was established. The decrease of current results from the competition of HRP labeled p24 antigen (HRP-p24) and p24 in serum samples to antip24 immobilized on the working electrode (WE), which leads to a linear decrease of the catalytic efficiency of H <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2</sub> O <sub xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2</sub> in p24 concentration ranges from 0.5 ~ 90 ng/mL with the detection limit of 0.2 ng/mL (3sigma). The proposed method needs neither separation nor washing up step, which simplifies the immunoassay procedure, shortens the analytical time, and provides a new promising platform to diagnosing the AIDs disease." @default.
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• W2080764575 date "2009-01-01" @default.
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• W2080764575 title "One Novel Nano Fe3O4-Au Modified Amperometric Immunosensor for Human Immunodeficiency Virus p24 Antigen" @default.
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• W2080764575 doi "https://doi.org/10.1109/bmei.2009.5305263" @default.
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