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• W2080768318 abstract "A simple microfluidic-based technique to quantitate the binding affinity between the glycopeptide antibiotics teicoplanin from Actinoplanes teicomyceticus and vancomycin from Streptomyces orientalis and 5-carboxyfluorescein-d-Ala-d-Ala-d-Ala (5-FAM-(DA)3) is described. In this work, (3-aminopropyl)triethoxysilane is used to modify the surfaces of a series of microchannels, and each channel is subsequently exposed to a solution of antibiotic for a few minutes. The antibiotic is retained after washing through electrostatic interactions, and the series of channels are subsequently exposed to an increasing concentration of 5-FAM-(DA)3 followed by washing to exclude any nonspecific binding. The extent of fluorescence is quantified using a microscope fitted with a CCD camera. The binding constants for the interaction of teicoplanin and vancomycin with the fluorescent peptide were determined to be 6.03 ± 0.97 × 104 and 4.93 ± 1.13 × 104 M−1, respectively, in good agreement with previous data. The ease of quantifying the extent of interaction in this microchip technique may prove powerful for exploration of a myriad of receptor−ligand pairs." @default.
• W2080768318 created "2016-06-24" @default.
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• W2080768318 date "2009-05-14" @default.
• W2080768318 modified "2023-09-25" @default.
• W2080768318 title "Development of Microfluidic Chips for Heterogeneous Receptor−Ligand Interaction Studies" @default.
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• W2080768318 doi "https://doi.org/10.1021/ac9006649" @default.
• W2080768318 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19441833" @default.
• W2080768318 hasPublicationYear "2009" @default.
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