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• W2080771911 abstract "The effect of graded concentrations of 2,4-dinitrophenol (DNP) on interrelationships between respiration and phosphorylations of Ehrlich ascites tumor cells was studied in vitro. DNP influence seemed to be mediated through two distinct mechanisms. One, coming into play at low DNP concentrations, effected the breakdown of labile and other acid-soluble phosphate esters in the cell, leading to increased concentrations of inorganic phosphorus in cells and medium. No pronounced impairment of inorganic phosphate uptake from medium into cells or of labile phosphorus formation within cells occurred, as evidenced by the continued incorporation of P32. Associated with this phase of DNP action, there was a rise in the rate of cellular respiration. As the concentration of DNP passed a certain threshold level, a second mode of action became apparent. Incorporation of P32 into cellular phosphate fractions, as well as respiration, became increasingly inhibited. Because of increased permeability of cells to inorganic and ester phosphorus at higher DNP concentrations and because of the inability of cells to regenerate labile phosphorus, it was suggested that the second phase of DNP action was associated with changes in the cell membrane. Incorporation of P32 into DNA-P was enhanced by a low concentration of DNP. There appeared to be some indication that cellular synthesis of DNA might be independently related to both the rate of O2 uptake and the availability of high-energy phosphorus." @default.
• W2080771911 created "2016-06-24" @default.
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• W2080771911 date "1955-08-01" @default.
• W2080771911 modified "2023-09-24" @default.
• W2080771911 title "Interrelations in respiratory, phosphorylative and mitotic activities of Ehrlich ascites tumor cells: Influence of dinitrophenol" @default.
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• W2080771911 doi "https://doi.org/10.1016/0003-9861(55)90301-5" @default.
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