Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080778144> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2080778144 endingPage "75" @default.
- W2080778144 startingPage "67" @default.
- W2080778144 abstract "The complete cleavage phase of V(D)J recombination includes four phases: binding of the active RAG complexes to the 12- or 23-signals, nicking of the signals, synapsis of the two signals, and hairpin formation at both signals concurrently. We have done time courses for the complete cleavage phase of the V(D)J recombination reaction and quantitated the amount of active RAG enzyme. We have also formulated a kinetic model for the binding, nicking, synapsis, and hairpin formation phases. We have utilized free solution enzymatic measurements for the binding and nicking phases as we do mathematical simulations of the kinetic model. This permits iteration of rate constants for the synapsis and hairpin formation phases until the model fits the observed overall cleavage time course. This process yields a rate constant for the hairpin formation that is 0.004 min(-1), which corresponds to an average catalytic cycle time of 250 min. This value is exceedingly close to a measured value of this constant that relied on wash-out of an inhibitory cofactor. The agreement indicates that this is likely to be the rate of the hairpin step over a wide range of range of conditions and irrespective of the DNA sequence of the V, D or J coding end located adjacent to the signal. These findings indicate that, under optimal in vitro conditions, the core RAG proteins carry out nicking at a rate which is nearly 150-fold faster than hairpin formation. The physiologic implications of this and other kinetic inferences of these time courses are discussed." @default.
- W2080778144 created "2016-06-24" @default.
- W2080778144 creator A5011827924 @default.
- W2080778144 creator A5020330256 @default.
- W2080778144 creator A5055737445 @default.
- W2080778144 creator A5079559642 @default.
- W2080778144 date "2004-01-01" @default.
- W2080778144 modified "2023-09-26" @default.
- W2080778144 title "Kinetic analysis of the nicking and hairpin formation steps in V(D)J recombination" @default.
- W2080778144 cites W1968821059 @default.
- W2080778144 cites W2003525376 @default.
- W2080778144 cites W2011392023 @default.
- W2080778144 cites W2011522975 @default.
- W2080778144 cites W2025592993 @default.
- W2080778144 cites W2034779212 @default.
- W2080778144 cites W2060453248 @default.
- W2080778144 cites W2103425962 @default.
- W2080778144 cites W2106660799 @default.
- W2080778144 cites W2108082319 @default.
- W2080778144 cites W2111435081 @default.
- W2080778144 cites W2120608812 @default.
- W2080778144 cites W2125622789 @default.
- W2080778144 cites W2131694075 @default.
- W2080778144 cites W2142405966 @default.
- W2080778144 cites W2145260507 @default.
- W2080778144 cites W2155375991 @default.
- W2080778144 cites W2160186676 @default.
- W2080778144 cites W2167863101 @default.
- W2080778144 cites W2170270573 @default.
- W2080778144 doi "https://doi.org/10.1016/j.dnarep.2003.09.006" @default.
- W2080778144 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14697761" @default.
- W2080778144 hasPublicationYear "2004" @default.
- W2080778144 type Work @default.
- W2080778144 sameAs 2080778144 @default.
- W2080778144 citedByCount "7" @default.
- W2080778144 countsByYear W20807781442014 @default.
- W2080778144 countsByYear W20807781442022 @default.
- W2080778144 crossrefType "journal-article" @default.
- W2080778144 hasAuthorship W2080778144A5011827924 @default.
- W2080778144 hasAuthorship W2080778144A5020330256 @default.
- W2080778144 hasAuthorship W2080778144A5055737445 @default.
- W2080778144 hasAuthorship W2080778144A5079559642 @default.
- W2080778144 hasConcept C104317684 @default.
- W2080778144 hasConcept C121332964 @default.
- W2080778144 hasConcept C12554922 @default.
- W2080778144 hasConcept C148898269 @default.
- W2080778144 hasConcept C151730666 @default.
- W2080778144 hasConcept C156695909 @default.
- W2080778144 hasConcept C175156509 @default.
- W2080778144 hasConcept C201437064 @default.
- W2080778144 hasConcept C2780254951 @default.
- W2080778144 hasConcept C43369102 @default.
- W2080778144 hasConcept C54355233 @default.
- W2080778144 hasConcept C552990157 @default.
- W2080778144 hasConcept C62520636 @default.
- W2080778144 hasConcept C86803240 @default.
- W2080778144 hasConceptScore W2080778144C104317684 @default.
- W2080778144 hasConceptScore W2080778144C121332964 @default.
- W2080778144 hasConceptScore W2080778144C12554922 @default.
- W2080778144 hasConceptScore W2080778144C148898269 @default.
- W2080778144 hasConceptScore W2080778144C151730666 @default.
- W2080778144 hasConceptScore W2080778144C156695909 @default.
- W2080778144 hasConceptScore W2080778144C175156509 @default.
- W2080778144 hasConceptScore W2080778144C201437064 @default.
- W2080778144 hasConceptScore W2080778144C2780254951 @default.
- W2080778144 hasConceptScore W2080778144C43369102 @default.
- W2080778144 hasConceptScore W2080778144C54355233 @default.
- W2080778144 hasConceptScore W2080778144C552990157 @default.
- W2080778144 hasConceptScore W2080778144C62520636 @default.
- W2080778144 hasConceptScore W2080778144C86803240 @default.
- W2080778144 hasIssue "1" @default.
- W2080778144 hasLocation W20807781441 @default.
- W2080778144 hasLocation W20807781442 @default.
- W2080778144 hasOpenAccess W2080778144 @default.
- W2080778144 hasPrimaryLocation W20807781441 @default.
- W2080778144 hasRelatedWork W2010709173 @default.
- W2080778144 hasRelatedWork W2015288239 @default.
- W2080778144 hasRelatedWork W2025592993 @default.
- W2080778144 hasRelatedWork W2035111151 @default.
- W2080778144 hasRelatedWork W2084354924 @default.
- W2080778144 hasRelatedWork W2108082319 @default.
- W2080778144 hasRelatedWork W2120608812 @default.
- W2080778144 hasRelatedWork W2140336226 @default.
- W2080778144 hasRelatedWork W3196221672 @default.
- W2080778144 hasRelatedWork W4245556281 @default.
- W2080778144 hasVolume "3" @default.
- W2080778144 isParatext "false" @default.
- W2080778144 isRetracted "false" @default.
- W2080778144 magId "2080778144" @default.
- W2080778144 workType "article" @default.