Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080804581> ?p ?o ?g. }
- W2080804581 endingPage "13192" @default.
- W2080804581 startingPage "13185" @default.
- W2080804581 abstract "Protein translocation in Escherichia coli is initiated by the interaction of a preprotein with the membrane translocase composed of a motor protein, SecA ATPase, and a membrane-embedded channel, the SecYEG complex. The extent to which the signal peptide region of the preprotein plays a role in SecYEG interactions is unclear, in part because studies in this area typically employ the entire preprotein. Using a synthetic signal peptide harboring a photoaffinity label in its hydrophobic core, we examined this interaction with SecYEG in a detergent micellar environment. The signal peptide was found to specifically bind SecY in a saturable manner and at levels comparable to those that stimulate SecA ATPase activity. Chemical and proteolytic cleavage of cross-linked SecY and analysis of the signal peptide adducts indicate that the binding was primarily to regions of the protein containing transmembrane domains seven and two. The signal peptide−SecY interaction was affected by the presence of SecA and nucleotides in a manner consistent with the transfer of signal peptide to SecY upon nucleotide hydrolysis at SecA." @default.
- W2080804581 created "2016-06-24" @default.
- W2080804581 creator A5020544332 @default.
- W2080804581 creator A5021223894 @default.
- W2080804581 creator A5030447237 @default.
- W2080804581 creator A5064861169 @default.
- W2080804581 date "2004-09-24" @default.
- W2080804581 modified "2023-09-23" @default.
- W2080804581 title "Demonstration of a Specific <i>Escherichia coli</i> SecY−Signal Peptide Interaction" @default.
- W2080804581 cites W106446473 @default.
- W2080804581 cites W127160757 @default.
- W2080804581 cites W1481524180 @default.
- W2080804581 cites W1506859180 @default.
- W2080804581 cites W1576501498 @default.
- W2080804581 cites W1590719173 @default.
- W2080804581 cites W161114408 @default.
- W2080804581 cites W1908462512 @default.
- W2080804581 cites W1964169934 @default.
- W2080804581 cites W1975085160 @default.
- W2080804581 cites W1976707563 @default.
- W2080804581 cites W1980750447 @default.
- W2080804581 cites W1981964231 @default.
- W2080804581 cites W1990024034 @default.
- W2080804581 cites W1991717719 @default.
- W2080804581 cites W1992800404 @default.
- W2080804581 cites W2008223866 @default.
- W2080804581 cites W2008415333 @default.
- W2080804581 cites W2013712639 @default.
- W2080804581 cites W2018289835 @default.
- W2080804581 cites W2021878227 @default.
- W2080804581 cites W2021911762 @default.
- W2080804581 cites W2022703044 @default.
- W2080804581 cites W2025941775 @default.
- W2080804581 cites W2031423487 @default.
- W2080804581 cites W2035306302 @default.
- W2080804581 cites W2040338718 @default.
- W2080804581 cites W2046876574 @default.
- W2080804581 cites W2048737734 @default.
- W2080804581 cites W2049701913 @default.
- W2080804581 cites W2052065771 @default.
- W2080804581 cites W2054816281 @default.
- W2080804581 cites W2055009712 @default.
- W2080804581 cites W2062509101 @default.
- W2080804581 cites W2066537459 @default.
- W2080804581 cites W2069088839 @default.
- W2080804581 cites W2073726493 @default.
- W2080804581 cites W2075027876 @default.
- W2080804581 cites W2077673149 @default.
- W2080804581 cites W2080304004 @default.
- W2080804581 cites W2083833295 @default.
- W2080804581 cites W2094624515 @default.
- W2080804581 cites W2096190827 @default.
- W2080804581 cites W2100837269 @default.
- W2080804581 cites W2102004032 @default.
- W2080804581 cites W2118278390 @default.
- W2080804581 cites W2121612894 @default.
- W2080804581 cites W2122524469 @default.
- W2080804581 cites W2126106174 @default.
- W2080804581 cites W2128635872 @default.
- W2080804581 cites W2144346115 @default.
- W2080804581 cites W2146626108 @default.
- W2080804581 cites W2149026054 @default.
- W2080804581 cites W2150079255 @default.
- W2080804581 cites W2150771080 @default.
- W2080804581 cites W2152354478 @default.
- W2080804581 cites W2152737402 @default.
- W2080804581 cites W2164358316 @default.
- W2080804581 cites W2324865313 @default.
- W2080804581 doi "https://doi.org/10.1021/bi049485k" @default.
- W2080804581 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3084660" @default.
- W2080804581 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15476412" @default.
- W2080804581 hasPublicationYear "2004" @default.
- W2080804581 type Work @default.
- W2080804581 sameAs 2080804581 @default.
- W2080804581 citedByCount "21" @default.
- W2080804581 countsByYear W20808045812014 @default.
- W2080804581 countsByYear W20808045812015 @default.
- W2080804581 countsByYear W20808045812017 @default.
- W2080804581 crossrefType "journal-article" @default.
- W2080804581 hasAuthorship W2080804581A5020544332 @default.
- W2080804581 hasAuthorship W2080804581A5021223894 @default.
- W2080804581 hasAuthorship W2080804581A5030447237 @default.
- W2080804581 hasAuthorship W2080804581A5064861169 @default.
- W2080804581 hasBestOaLocation W20808045812 @default.
- W2080804581 hasConcept C104317684 @default.
- W2080804581 hasConcept C10858879 @default.
- W2080804581 hasConcept C113035283 @default.
- W2080804581 hasConcept C115335371 @default.
- W2080804581 hasConcept C12554922 @default.
- W2080804581 hasConcept C138626823 @default.
- W2080804581 hasConcept C141315368 @default.
- W2080804581 hasConcept C144647389 @default.
- W2080804581 hasConcept C167625842 @default.
- W2080804581 hasConcept C170493617 @default.
- W2080804581 hasConcept C181199279 @default.
- W2080804581 hasConcept C185592680 @default.
- W2080804581 hasConcept C23265538 @default.
- W2080804581 hasConcept C24530287 @default.