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- W2080852357 abstract "A simple method for the preparation of fully protected 2′-deoxynucleoside-3′-phosphates is described. The main step, phosphorylation of partially protected deoxynucleosides, is performed with the monofunctional reagent p-chlorophenyl (2-cyanoethyl) phosphochloridate3) (2) in dioxane. This reagent is quickly prepared from readily accessible p-chlorophenyl phosphodichloridate by reaction with 3-hydroxypropionitrile and triethylamine in dioxane or ether. The crude reagent is used directly for phosphorylation. After chromatography, protected deoxynucleoside-3′-phosphates were isolated in yields of 80–87%. The method described here, which has been optimized in detail, represents a simple alternative to published methods." @default.
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- W2080852357 date "1981-11-04" @default.
- W2080852357 modified "2023-09-27" @default.
- W2080852357 title "Nucleosides and Nucleotides. Part 17. A simple preparation of protected deoxynucleoside-3'-phosphates" @default.
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- W2080852357 doi "https://doi.org/10.1002/hlca.19810640719" @default.
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