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• W2080852499 abstract "The substrate preferences of three acylases – two wild-type enzymes and an evolved variant obtained by directed evolution – which are prototypical enzymes for glutaryl-7-ACA acylase and cephalosporin C acylase subfamilies, have been investigated. A preliminary screening of enzymes’ performances on a large set of substrates has been carried out by a colorimetric assay performed in 96-well plates and by a pH-Stat monitoring the hydrolytic activities. Subsequently, kinetic data for selected substrates have been determined, thus elucidating the substrate preference of members of glutaryl-7-ACA acylase vs. cephalosporin C acylase subfamilies. These achievements pave the way to the ability of choosing the best enzyme for the hydrolysis of different compounds of industrial importance." @default.
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• W2080852499 date "2012-04-01" @default.
• W2080852499 modified "2023-10-03" @default.
• W2080852499 title "On the substrate preference of glutaryl acylases" @default.
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• W2080852499 doi "https://doi.org/10.1016/j.molcatb.2011.12.001" @default.
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