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- W2080853969 abstract "Summary Isolated leaves of Lobularia maritima floating on a 0.2 M NaCl solution for 68 hours were induced to succulence as determined by doubling of leaf thickness and leaf fresh weight due to increases in cell volume of palisade and sponge parenchyma cells. Control leaves floating on water showed a 30% increase in wet weight due to water uptake and only minimal thickening. The absolute DNA content per leaf (but not the DNA content per gram of leaf tissue) increased by 30% over the DNA content of control leaves. This increase is due to the increase of the proportion of individual nuclei with increased nuclear DNA contents as determined by ethidium bromide fluorescence measurements. The DNA increase is mainly due to the selective synthesis of a DNA fraction with high GC content which shows as a separate peak with melting temperature 95.5°C in the thermal denaturation profile in 0.12 M phosphate buffer and a separate band (1.723 g cm-3) in the buoyant density profile in neutral CsCl gradient centrifugation. The high GC fraction can be separated preparatively from main band DNA by Ag+/Cs2SO4 centrifugation." @default.
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- W2080853969 date "1983-05-01" @default.
- W2080853969 modified "2023-09-26" @default.
- W2080853969 title "Changes of Nuclear DNA Composition After Induction of Succulence in Lobularia maritima" @default.
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- W2080853969 doi "https://doi.org/10.1016/s0044-328x(83)80108-1" @default.
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