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- W2080863958 abstract "The synthesis of α-fetoprotein and other fetal plasma proteins was studied by perfusion of isolated human fetal livers with an artificial medium containing leucine-1-14C. The rates of release by fetal liver of α-fetoprotein and albumin were analyzed by measuring the changes in their concentrations in the perfusion medium after the first hour of perfusion. The release of α-fetoprotein from the liver of a 14-week fetus was 19 μg/min. At 17 weeks of gestation, the rate was 26 μg/min and, at 20 weeks, 21 μg/min. The corresponding values for albumin were 50 μg/min, 92 μ/min, and 196 μ/min. Within the same period of gestation, the plasma concentration of α-fetoprotein decreased and that of albumin increased with advancing fetal age. The results suggest a more rapid turnover of α-fetoprotein than albumin in vivo. The incorporation of leucine-1-14Cinto serum proteins was determined by autoradiography after electrophoresis on cellulose acetate, immunoelectrophoresis, or immunodiffusion. Radioactivity was found in α-fetoprotein, albumin, α1-antitrypsin, Gc-protein and α-lipoprotein — indicating hepatic synthesis of these proteins. No radioactivity was found in IgG present in the perfusion medium, and no haptoglobin was found in the medium." @default.
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- W2080863958 date "1971-09-15" @default.
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- W2080863958 title "Perfusion of isolated human fetal liver: Synthesis and release of α-fetoprotein and albumin" @default.
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- W2080863958 doi "https://doi.org/10.1002/ijc.2910080209" @default.
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