FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080863990> ?p ?o ?g. }

• W2080863990 endingPage "234" @default.
• W2080863990 startingPage "230" @default.
• W2080863990 abstract "A telomerase assay has been developed for high-throughput screening in 96-well microtiter plates. A crude cell lysate which adds telomere repeats to a biotinylated DNA primer is the source of telomerase. The telomerase-extended primer is hybridized to a digoxigenin-labeled telomere antisense DNA probe. The hybrid is further processed by enzyme-linked immunosorbent assay (ELISA) as follows. The biotinylated hybrid is captured on streptavidin-coated microtiter plates. The immobilized hybrid is probed with alkaline phosphatase-antidigoxigenin and detected via chemiluminescent readout. The limit of detection of a chemically synthesized tetra-telomere repeat was about 10 attomoles. Apparent telomerase activity was detected in lysates of 293T cells. The signal to background for the assay (ratio of signal for the complete assay mixture divided by the signal for the assay mixture without primer) was around 10. An automated system that performed unattended runs of up to 17 96-well microtiter plates in 8h was constructed." @default.
• W2080863990 created "2016-06-24" @default.
• W2080863990 creator A5014769441 @default.
• W2080863990 creator A5016517074 @default.
• W2080863990 creator A5020507413 @default.
• W2080863990 creator A5027193322 @default.
• W2080863990 creator A5037958863 @default.
• W2080863990 creator A5040434682 @default.
• W2080863990 creator A5040686568 @default.
• W2080863990 creator A5055056719 @default.
• W2080863990 creator A5061008819 @default.
• W2080863990 creator A5080285568 @default.
• W2080863990 creator A5089222740 @default.
• W2080863990 date "2004-08-01" @default.
• W2080863990 modified "2023-10-14" @default.
• W2080863990 title "A telomerase enzymatic assay that does not use polymerase chain reaction, radioactivity, or electrophoresis" @default.
• W2080863990 cites W1990950933 @default.
• W2080863990 cites W1991001144 @default.
• W2080863990 cites W2000971601 @default.
• W2080863990 cites W2014482493 @default.
• W2080863990 cites W2074245628 @default.
• W2080863990 cites W2074631079 @default.
• W2080863990 cites W2091741557 @default.
• W2080863990 cites W2119048840 @default.
• W2080863990 cites W2144118457 @default.
• W2080863990 cites W2145344158 @default.
• W2080863990 cites W4254759378 @default.
• W2080863990 doi "https://doi.org/10.1016/j.ab.2004.03.073" @default.
• W2080863990 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15265727" @default.
• W2080863990 hasPublicationYear "2004" @default.
• W2080863990 type Work @default.
• W2080863990 sameAs 2080863990 @default.
• W2080863990 citedByCount "19" @default.
• W2080863990 countsByYear W20808639902012 @default.
• W2080863990 countsByYear W20808639902013 @default.
• W2080863990 countsByYear W20808639902014 @default.
• W2080863990 countsByYear W20808639902015 @default.
• W2080863990 countsByYear W20808639902016 @default.
• W2080863990 countsByYear W20808639902017 @default.
• W2080863990 countsByYear W20808639902018 @default.
• W2080863990 countsByYear W20808639902019 @default.
• W2080863990 countsByYear W20808639902020 @default.
• W2080863990 countsByYear W20808639902021 @default.
• W2080863990 crossrefType "journal-article" @default.
• W2080863990 hasAuthorship W2080863990A5014769441 @default.
• W2080863990 hasAuthorship W2080863990A5016517074 @default.
• W2080863990 hasAuthorship W2080863990A5020507413 @default.
• W2080863990 hasAuthorship W2080863990A5027193322 @default.
• W2080863990 hasAuthorship W2080863990A5037958863 @default.
• W2080863990 hasAuthorship W2080863990A5040434682 @default.
• W2080863990 hasAuthorship W2080863990A5040686568 @default.
• W2080863990 hasAuthorship W2080863990A5055056719 @default.
• W2080863990 hasAuthorship W2080863990A5061008819 @default.
• W2080863990 hasAuthorship W2080863990A5080285568 @default.
• W2080863990 hasAuthorship W2080863990A5089222740 @default.
• W2080863990 hasConcept C104317684 @default.
• W2080863990 hasConcept C119128265 @default.
• W2080863990 hasConcept C153911025 @default.
• W2080863990 hasConcept C177336024 @default.
• W2080863990 hasConcept C178790620 @default.
• W2080863990 hasConcept C185592680 @default.
• W2080863990 hasConcept C204628709 @default.
• W2080863990 hasConcept C2777563447 @default.
• W2080863990 hasConcept C2778810118 @default.
• W2080863990 hasConcept C43617362 @default.
• W2080863990 hasConcept C552990157 @default.
• W2080863990 hasConcept C55493867 @default.
• W2080863990 hasConcept C86803240 @default.
• W2080863990 hasConcept C94581717 @default.
• W2080863990 hasConceptScore W2080863990C104317684 @default.
• W2080863990 hasConceptScore W2080863990C119128265 @default.
• W2080863990 hasConceptScore W2080863990C153911025 @default.
• W2080863990 hasConceptScore W2080863990C177336024 @default.
• W2080863990 hasConceptScore W2080863990C178790620 @default.
• W2080863990 hasConceptScore W2080863990C185592680 @default.
• W2080863990 hasConceptScore W2080863990C204628709 @default.
• W2080863990 hasConceptScore W2080863990C2777563447 @default.
• W2080863990 hasConceptScore W2080863990C2778810118 @default.
• W2080863990 hasConceptScore W2080863990C43617362 @default.
• W2080863990 hasConceptScore W2080863990C552990157 @default.
• W2080863990 hasConceptScore W2080863990C55493867 @default.
• W2080863990 hasConceptScore W2080863990C86803240 @default.
• W2080863990 hasConceptScore W2080863990C94581717 @default.
• W2080863990 hasIssue "2" @default.
• W2080863990 hasLocation W20808639901 @default.
• W2080863990 hasLocation W20808639902 @default.
• W2080863990 hasOpenAccess W2080863990 @default.
• W2080863990 hasPrimaryLocation W20808639901 @default.
• W2080863990 hasRelatedWork W1550018201 @default.
• W2080863990 hasRelatedWork W1975347381 @default.
• W2080863990 hasRelatedWork W2017948235 @default.
• W2080863990 hasRelatedWork W2058280701 @default.
• W2080863990 hasRelatedWork W2074863155 @default.
• W2080863990 hasRelatedWork W2080863990 @default.
• W2080863990 hasRelatedWork W2143754645 @default.
• W2080863990 hasRelatedWork W2355813221 @default.
• W2080863990 hasRelatedWork W2969967023 @default.
• W2080863990 hasRelatedWork W3130033876 @default.

• next