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- W2080872743 abstract "The beneficial properties of human milk oligosaccharides (OS) are largely due to their fermentation by the infant's colonic microflora. Aim: To study the fermentation pattern of human milk OS (HMOS) in vitro, and to compare it to the fermentation of OS (to be) used in infant nutrition. Methods: An in vitro semi-dynamic fermentation system was inoculated with fresh faeces from either breast-fed or formula-fed infants (age <1 yr). HMOS, a mixture of galacto- & long-chain fructo-OS (GOS/FOS, 9:1), acidic OS (AOS, derived from citrus pectin; equivalent to carrot soup OS) and intact citrus pectin were added and samples were taken at t = 3, 24 and 48 hr. Samples were analysed for short-chain fatty acids (SCFA) by GC. Gas production was also measured. In every experiment, glucose fermentations were carried out as a reference. Results: Total SCFA and gas production are expressed as percentage relative to glucose (mean ± SEM). Molar ratio SCFA = acetate: propionate: butyrate.TableIn a separate experiment using faeces from a formula-fed infant with similar results for GOS/FOS, AOS produced more SCFA and gas than intact citrus pectin, but with an identical SCFA ratio. Summary & Conclusions: GOS/FOS are fermented gradually and almost completely by the colonic microflora of breast-fed and formula-fed infants, in a similar way to HMOS. AOS are also well fermented by the infant's microflora: (a) initially slower than GOS/FOS, but with a higher final gas production, (b) more completely than intact citrus pectin, and (c) with an identical SCFA ratio." @default.
- W2080872743 created "2016-06-24" @default.
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- W2080872743 date "2005-05-01" @default.
- W2080872743 modified "2023-09-26" @default.
- W2080872743 title "COMPARISON OF HUMAN MILK OLIGOSACCHARIDES WITH OLIGOSACCHARIDES FOR USE IN INFANT NUTRITION ON IN VITRO FERMENTATION USING FAECES FROM BREAST-FED AND FORMULA-FED INFANTS" @default.
- W2080872743 doi "https://doi.org/10.1097/00005176-200505000-00238" @default.
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