FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080885576> ?p ?o ?g. }

• W2080885576 endingPage "e8288" @default.
• W2080885576 startingPage "e8288" @default.
• W2080885576 abstract "Deliberate and natural outbreaks of infectious disease underscore the necessity of effective vaccines and antimicrobial/antiviral therapeutics. The prevalence of antibiotic resistant strains and the ease by which antibiotic resistant bacteria can be intentionally engineered further highlights the need for continued development of novel antibiotics against new bacterial targets. Isoprenes are a class of molecules fundamentally involved in a variety of crucial biological functions. Mammalian cells utilize the mevalonic acid pathway for isoprene biosynthesis, whereas many bacteria utilize the methylerythritol phosphate (MEP) pathway, making the latter an attractive target for antibiotic development. In this report we describe the cloning and characterization of Francisella tularensis MEP synthase, a MEP pathway enzyme and potential target for antibiotic development. In vitro growth-inhibition assays using fosmidomycin, an inhibitor of MEP synthase, illustrates the effectiveness of MEP pathway inhibition with F. tularensis. To facilitate drug development, F. tularensis MEP synthase was cloned, expressed, purified, and characterized. Enzyme assays produced apparent kinetic constants (K(M)(DXP) = 104 microM, K(M)(NADPH) = 13 microM, k(cat)(DXP) = 2 s(-1), k(cat)(NADPH) = 1.3 s(-1)), an IC(50) for fosmidomycin of 247 nM, and a K(i) for fosmidomycin of 99 nM. The enzyme exhibits a preference for Mg(+2) as a divalent cation. Titanium dioxide chromatography-tandem mass spectrometry identified Ser177 as a site of phosphorylation. S177D and S177E site-directed mutants are inactive, suggesting a mechanism for post-translational control of metabolic flux through the F. tularensis MEP pathway. Overall, our study suggests that MEP synthase is an excellent target for the development of novel antibiotics against F. tularensis." @default.
• W2080885576 created "2016-06-24" @default.
• W2080885576 creator A5003120669 @default.
• W2080885576 creator A5013069852 @default.
• W2080885576 creator A5017999390 @default.
• W2080885576 creator A5019810138 @default.
• W2080885576 creator A5023275344 @default.
• W2080885576 creator A5042726594 @default.
• W2080885576 creator A5044882491 @default.
• W2080885576 creator A5085616789 @default.
• W2080885576 date "2009-12-14" @default.
• W2080885576 modified "2023-10-16" @default.
• W2080885576 title "Kinetic Characterization and Phosphoregulation of the Francisella tularensis 1-Deoxy-D-Xylulose 5-Phosphate Reductoisomerase (MEP Synthase)" @default.
• W2080885576 cites W1272861888 @default.
• W2080885576 cites W1513122208 @default.
• W2080885576 cites W1572067593 @default.
• W2080885576 cites W1924794236 @default.
• W2080885576 cites W1968547228 @default.
• W2080885576 cites W1976725431 @default.
• W2080885576 cites W1977444198 @default.
• W2080885576 cites W1987474460 @default.
• W2080885576 cites W1990286749 @default.
• W2080885576 cites W1991001144 @default.
• W2080885576 cites W1997637252 @default.
• W2080885576 cites W1999097194 @default.
• W2080885576 cites W2003219159 @default.
• W2080885576 cites W2007256796 @default.
• W2080885576 cites W2008872195 @default.
• W2080885576 cites W2012632418 @default.
• W2080885576 cites W2015946472 @default.
• W2080885576 cites W2020003403 @default.
• W2080885576 cites W2028539126 @default.
• W2080885576 cites W2031612794 @default.
• W2080885576 cites W2036479712 @default.
• W2080885576 cites W2042044881 @default.
• W2080885576 cites W2042784771 @default.
• W2080885576 cites W2045132607 @default.
• W2080885576 cites W2053635523 @default.
• W2080885576 cites W2058639789 @default.
• W2080885576 cites W2059007103 @default.
• W2080885576 cites W2063049515 @default.
• W2080885576 cites W2067901702 @default.
• W2080885576 cites W2067944903 @default.
• W2080885576 cites W2068066782 @default.
• W2080885576 cites W2068092064 @default.
• W2080885576 cites W2069030760 @default.
• W2080885576 cites W2071124826 @default.
• W2080885576 cites W2076480832 @default.
• W2080885576 cites W2078178981 @default.
• W2080885576 cites W2080172904 @default.
• W2080885576 cites W2081290361 @default.
• W2080885576 cites W2091449708 @default.
• W2080885576 cites W2093637194 @default.
• W2080885576 cites W2094714681 @default.
• W2080885576 cites W2105069516 @default.
• W2080885576 cites W2114276495 @default.
• W2080885576 cites W2122228499 @default.
• W2080885576 cites W2126109293 @default.
• W2080885576 cites W2142251083 @default.
• W2080885576 cites W2142801964 @default.
• W2080885576 cites W2149092807 @default.
• W2080885576 cites W2152274865 @default.
• W2080885576 cites W2172191471 @default.
• W2080885576 cites W2318033222 @default.
• W2080885576 cites W2325749680 @default.
• W2080885576 cites W2329369835 @default.
• W2080885576 cites W2038045361 @default.
• W2080885576 cites W2470795803 @default.
• W2080885576 doi "https://doi.org/10.1371/journal.pone.0008288" @default.
• W2080885576 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2788227" @default.
• W2080885576 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20011597" @default.
• W2080885576 hasPublicationYear "2009" @default.
• W2080885576 type Work @default.
• W2080885576 sameAs 2080885576 @default.
• W2080885576 citedByCount "32" @default.
• W2080885576 countsByYear W20808855762012 @default.
• W2080885576 countsByYear W20808855762013 @default.
• W2080885576 countsByYear W20808855762014 @default.
• W2080885576 countsByYear W20808855762015 @default.
• W2080885576 countsByYear W20808855762016 @default.
• W2080885576 countsByYear W20808855762018 @default.
• W2080885576 countsByYear W20808855762019 @default.
• W2080885576 countsByYear W20808855762020 @default.
• W2080885576 countsByYear W20808855762021 @default.
• W2080885576 countsByYear W20808855762022 @default.
• W2080885576 countsByYear W20808855762023 @default.
• W2080885576 crossrefType "journal-article" @default.
• W2080885576 hasAuthorship W2080885576A5003120669 @default.
• W2080885576 hasAuthorship W2080885576A5013069852 @default.
• W2080885576 hasAuthorship W2080885576A5017999390 @default.
• W2080885576 hasAuthorship W2080885576A5019810138 @default.
• W2080885576 hasAuthorship W2080885576A5023275344 @default.
• W2080885576 hasAuthorship W2080885576A5042726594 @default.
• W2080885576 hasAuthorship W2080885576A5044882491 @default.
• W2080885576 hasAuthorship W2080885576A5085616789 @default.
• W2080885576 hasBestOaLocation W20808855761 @default.
• W2080885576 hasConcept C104317684 @default.
• W2080885576 hasConcept C112243037 @default.

• next