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- W2080905770 abstract "A full-length cDNA for cysteine-rich venom protein (CRVP) was constructed by immunoscreening and 5′-rapid amplification of cDNA ends from a cDNA library of venom gland of Trimeresurus mucrosquamatus. The predicted CRVP consisted of 183 amino acid residues including a putative signal peptide of 21 residues. Northern blot hybridization suggested the tissue-specific expression in venom gland and its corresponding length of cDNA. The predicted amino acid sequence of CRVP was homologous to a rat epididymal metalloprotein and a lizard helothermine. Amino acid sequence analysis suggested that CRVP may be a venom metallo-protein targeted against ryanodine receptors and Ca2+ release. Moreover, CRVP expressed in Escherichia coli exhibited the same antigenicity as their native venom forms of T. mucrosquamatus. This is the first report in the cloning and expression of a CRVP from the venom gland of T. mucrosquamatus." @default.
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- W2080905770 date "1997-06-01" @default.
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- W2080905770 title "Cloning and expression of a cysteine-rich venom protein from Trimeresurus mucrosquamatus (Taiwan Habu)" @default.
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- W2080905770 doi "https://doi.org/10.1016/s0041-0101(96)00192-4" @default.
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