FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080930010> ?p ?o ?g. }

• W2080930010 endingPage "110" @default.
• W2080930010 startingPage "102" @default.
• W2080930010 abstract "The prokaryotic Cre-loxP recombination system is a powerful tool that enables in vitro and in vivo site-specific manipulations of the genome of eukaryotic cells as well as of DNA viruses and their derived vectors. This system, however, has not yet been exploited in the context of herpes simplex virus type 1 (HSV-1) infected cells, perhaps because this virus encodes several functions that induce a strong shutoff of cellular protein synthesis, a fact that could preclude expression of cellular-encoded Cre recombinase. In the present study, we show that efficient site-specific recombination can take place in cell lines expressing Cre, even in the context of HSV-1 infection, as evidenced by the engineering of an HSV-1 recombinant virus and several viral vectors carrying one or two loxP sequences. More precisely, we have used this system to induce an irreversible switch in the expression of a viral complex transcription unit encoding two different open reading frames and allowing consecutive expression of two reporter genes. Furthermore Cre recombinations were also used to induce the decatenation of the genomic concatemers harbored by amplicon particles upon infection of cells under nonreplicative conditions, thus enabling the rescue of many independent plasmids corresponding to the original amplicon plasmid used to generate the vectors. Thus the Cre-loxP recombination system can successfully be used for engineering the genome of HSV-1 or HSV-1-based vectors in cultured cells." @default.
• W2080930010 created "2016-06-24" @default.
• W2080930010 creator A5040235486 @default.
• W2080930010 creator A5081220247 @default.
• W2080930010 date "2000-02-01" @default.
• W2080930010 modified "2023-09-26" @default.
• W2080930010 title "Genetic Engineering of Herpes Simplex Virus and Vector Genomes Carrying loxP Sites in Cells Expressing Cre Recombinase" @default.
• W2080930010 cites W124775406 @default.
• W2080930010 cites W1490731141 @default.
• W2080930010 cites W1508904610 @default.
• W2080930010 cites W1675494033 @default.
• W2080930010 cites W1965372133 @default.
• W2080930010 cites W1967287827 @default.
• W2080930010 cites W1968605428 @default.
• W2080930010 cites W1984707261 @default.
• W2080930010 cites W1989790258 @default.
• W2080930010 cites W1991711092 @default.
• W2080930010 cites W1997273754 @default.
• W2080930010 cites W2003605588 @default.
• W2080930010 cites W2053200644 @default.
• W2080930010 cites W2060020224 @default.
• W2080930010 cites W2075921029 @default.
• W2080930010 cites W2094853557 @default.
• W2080930010 cites W2107902950 @default.
• W2080930010 cites W2120412445 @default.
• W2080930010 cites W2145275694 @default.
• W2080930010 cites W2148559405 @default.
• W2080930010 cites W2161738023 @default.
• W2080930010 cites W4246387564 @default.
• W2080930010 doi "https://doi.org/10.1006/viro.1999.0108" @default.
• W2080930010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10648187" @default.
• W2080930010 hasPublicationYear "2000" @default.
• W2080930010 type Work @default.
• W2080930010 sameAs 2080930010 @default.
• W2080930010 citedByCount "18" @default.
• W2080930010 countsByYear W20809300102012 @default.
• W2080930010 countsByYear W20809300102013 @default.
• W2080930010 countsByYear W20809300102015 @default.
• W2080930010 crossrefType "journal-article" @default.
• W2080930010 hasAuthorship W2080930010A5040235486 @default.
• W2080930010 hasAuthorship W2080930010A5081220247 @default.
• W2080930010 hasBestOaLocation W20809300101 @default.
• W2080930010 hasConcept C102230213 @default.
• W2080930010 hasConcept C102414288 @default.
• W2080930010 hasConcept C104317684 @default.
• W2080930010 hasConcept C125929170 @default.
• W2080930010 hasConcept C141035611 @default.
• W2080930010 hasConcept C141231307 @default.
• W2080930010 hasConcept C153911025 @default.
• W2080930010 hasConcept C156695909 @default.
• W2080930010 hasConcept C159047783 @default.
• W2080930010 hasConcept C22744801 @default.
• W2080930010 hasConcept C2522874641 @default.
• W2080930010 hasConcept C2781196997 @default.
• W2080930010 hasConcept C38027674 @default.
• W2080930010 hasConcept C39060996 @default.
• W2080930010 hasConcept C40767141 @default.
• W2080930010 hasConcept C49105822 @default.
• W2080930010 hasConcept C54355233 @default.
• W2080930010 hasConcept C8185291 @default.
• W2080930010 hasConcept C86803240 @default.
• W2080930010 hasConcept C92087593 @default.
• W2080930010 hasConceptScore W2080930010C102230213 @default.
• W2080930010 hasConceptScore W2080930010C102414288 @default.
• W2080930010 hasConceptScore W2080930010C104317684 @default.
• W2080930010 hasConceptScore W2080930010C125929170 @default.
• W2080930010 hasConceptScore W2080930010C141035611 @default.
• W2080930010 hasConceptScore W2080930010C141231307 @default.
• W2080930010 hasConceptScore W2080930010C153911025 @default.
• W2080930010 hasConceptScore W2080930010C156695909 @default.
• W2080930010 hasConceptScore W2080930010C159047783 @default.
• W2080930010 hasConceptScore W2080930010C22744801 @default.
• W2080930010 hasConceptScore W2080930010C2522874641 @default.
• W2080930010 hasConceptScore W2080930010C2781196997 @default.
• W2080930010 hasConceptScore W2080930010C38027674 @default.
• W2080930010 hasConceptScore W2080930010C39060996 @default.
• W2080930010 hasConceptScore W2080930010C40767141 @default.
• W2080930010 hasConceptScore W2080930010C49105822 @default.
• W2080930010 hasConceptScore W2080930010C54355233 @default.
• W2080930010 hasConceptScore W2080930010C8185291 @default.
• W2080930010 hasConceptScore W2080930010C86803240 @default.
• W2080930010 hasConceptScore W2080930010C92087593 @default.
• W2080930010 hasIssue "1" @default.
• W2080930010 hasLocation W20809300101 @default.
• W2080930010 hasLocation W20809300102 @default.
• W2080930010 hasOpenAccess W2080930010 @default.
• W2080930010 hasPrimaryLocation W20809300101 @default.
• W2080930010 hasRelatedWork W1024660077 @default.
• W2080930010 hasRelatedWork W1970057644 @default.
• W2080930010 hasRelatedWork W1997816780 @default.
• W2080930010 hasRelatedWork W2023089872 @default.
• W2080930010 hasRelatedWork W2066736245 @default.
• W2080930010 hasRelatedWork W2080930010 @default.
• W2080930010 hasRelatedWork W2142920348 @default.
• W2080930010 hasRelatedWork W2165613721 @default.
• W2080930010 hasRelatedWork W2407030612 @default.
• W2080930010 hasRelatedWork W2976520252 @default.
• W2080930010 hasVolume "267" @default.

• next