FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080940688> ?p ?o ?g. }

• W2080940688 abstract "As a natural defense mechanism of the immune system, a group of white blood cells known as B cells produce antibodies to neutralize and clear pathogens and toxins from circulation. In order to increase the antibody binding strength, B cells undergo two types of genomic alterations: somatic hypermutation and class switch recombination (CSR). CSR is accomplished by the B cell-specific activation-induced cytidine deaminase protein (AID) which allows activated B cells to produce antibodies of different isotypes, which have distinct effector functions. Current research concerning the nature of antibody diversification examines the role of specific proteins in the CSR process. To test the role of these genes in CSR, I cloned the cDNAs of these genes. Through polymerase chain reaction (PCR) and PCR clean-up, the PCR products were ligated into an expression vector that harbours a drug-resistant gene. A variety of primers including RNF20 ’UTR, RNF40 ‘UTR, RNF40 clone and RNF40 clone targeted cDNA samples through gradient PCR. Although electrophoresis analyses show inconsistency with PCR products, conclusions can be drawn about the efficiency of primers, Phusion, and cDNA samples. Successful PCR products will be expressed in the CH12F3-2 B cell line that undergoes CSR at high rates and assess whether the CSR process is affected. I will also knockdown RNF20 and RNF40 by siRNA. If the hypothesis is correct, RNF20 and RNF40 will be discovered to be critical factors in the CSR process in B cells, and therefore in establishing an effective antibody response that is critical in protective immunity. En tant qu’un mecanisme de defense naturel du systeme immunitaire, un groupe de globules blancs appeles lymphocytes B produisent des anticorps afin de neutraliser et eliminer les agents pathogenes et les toxines de la circulation. Afin d’augmenter la force de liaison des anticorps, les cellules B subissent deux types d’alterations genomiques: une hypermutation somatique et une commutation de classe des entreprises (RSE). La RSE se realise grâce a l’activation induite des proteines cytidine desaminase (AID) specifique aux cellules B, qui permet aux cellules B activees de produire des anticorps de differents isotypes detenant des fonctions effectrices differentes. La recherche actuelle concernant la nature diversifiee d’anticorps examine le role de proteines specifiques dans le processus RSE. Afin de tester le role de ces genes dans RSE, j’ai clone l’ADNc dans ces genes. Par reaction en chaine par polymerase (PCR) et la purification PCR, les produits obtenus par PCR ont subi une ligature dans un vecteur d’expression qui abrite un gene resistant aux medicaments. Une variete d'amorces don RNF20 ’UTR, RNF40‘UTR, le clone RNF40 et le clone RNF40 ciblaient des echantillons d’ADNc par un gradient PCR. Bien que les analyses d'electrophorese montrent une certaine incompatibilite avec les produits de la PCR, certaines conclusions au sujet de l’efficacite des echantillons d’amorces, de Physion et de l’ADNc peuvent en etre tirees. Les produits de la PCR retenus seront exprimes dans la lignee cellulaire CH12F3-2 B qui subit la RSE a des taux eleves pour ensuite evaluer si la demarche RSE est affectee. Je vais aussi effectuer un choc sur les genes RNF20 et RNF40 par ARNsi. Si l'hypothese est juste, le RNF20 et RNF40 seront identifies en tant que facteurs critiques dans le processus RSE dans les cellules B, et donc critiques dans l’etablissement d’une reponse d’anticorps efficace de l'immunite protectrice." @default.
• W2080940688 created "2016-06-24" @default.
• W2080940688 creator A5081640577 @default.
• W2080940688 date "2013-09-01" @default.
• W2080940688 modified "2023-09-24" @default.
• W2080940688 title "Characterizing RNF20 and RNF40 in the Class Switching of B Cells1" @default.
• W2080940688 cites W1491459594 @default.
• W2080940688 cites W2043870412 @default.
• W2080940688 cites W2052532932 @default.
• W2080940688 cites W2059183599 @default.
• W2080940688 cites W2167333291 @default.
• W2080940688 cites W2465591554 @default.
• W2080940688 cites W652536951 @default.
• W2080940688 doi "https://doi.org/10.13034/cysj-2013-007" @default.
• W2080940688 hasPublicationYear "2013" @default.
• W2080940688 type Work @default.
• W2080940688 sameAs 2080940688 @default.
• W2080940688 citedByCount "0" @default.
• W2080940688 crossrefType "journal-article" @default.
• W2080940688 hasAuthorship W2080940688A5081640577 @default.
• W2080940688 hasBestOaLocation W20809406881 @default.
• W2080940688 hasConcept C104317684 @default.
• W2080940688 hasConcept C128526571 @default.
• W2080940688 hasConcept C137756551 @default.
• W2080940688 hasConcept C153911025 @default.
• W2080940688 hasConcept C159654299 @default.
• W2080940688 hasConcept C173396325 @default.
• W2080940688 hasConcept C187882448 @default.
• W2080940688 hasConcept C2776985229 @default.
• W2080940688 hasConcept C2778453870 @default.
• W2080940688 hasConcept C54355233 @default.
• W2080940688 hasConcept C86803240 @default.
• W2080940688 hasConceptScore W2080940688C104317684 @default.
• W2080940688 hasConceptScore W2080940688C128526571 @default.
• W2080940688 hasConceptScore W2080940688C137756551 @default.
• W2080940688 hasConceptScore W2080940688C153911025 @default.
• W2080940688 hasConceptScore W2080940688C159654299 @default.
• W2080940688 hasConceptScore W2080940688C173396325 @default.
• W2080940688 hasConceptScore W2080940688C187882448 @default.
• W2080940688 hasConceptScore W2080940688C2776985229 @default.
• W2080940688 hasConceptScore W2080940688C2778453870 @default.
• W2080940688 hasConceptScore W2080940688C54355233 @default.
• W2080940688 hasConceptScore W2080940688C86803240 @default.
• W2080940688 hasLocation W20809406881 @default.
• W2080940688 hasOpenAccess W2080940688 @default.
• W2080940688 hasPrimaryLocation W20809406881 @default.
• W2080940688 hasRelatedWork W149342241 @default.
• W2080940688 hasRelatedWork W2723744440 @default.
• W2080940688 hasRelatedWork W2801116860 @default.
• W2080940688 hasRelatedWork W2994852054 @default.
• W2080940688 hasRelatedWork W2999746281 @default.
• W2080940688 hasRelatedWork W3032899870 @default.
• W2080940688 hasRelatedWork W3033906386 @default.
• W2080940688 hasRelatedWork W3043189883 @default.
• W2080940688 hasRelatedWork W3096797963 @default.
• W2080940688 hasRelatedWork W3109808250 @default.
• W2080940688 hasRelatedWork W3117944704 @default.
• W2080940688 hasRelatedWork W3133675974 @default.
• W2080940688 hasRelatedWork W3138318811 @default.
• W2080940688 hasRelatedWork W3161017700 @default.
• W2080940688 hasRelatedWork W3162480930 @default.
• W2080940688 hasRelatedWork W3164024152 @default.
• W2080940688 hasRelatedWork W3186602782 @default.
• W2080940688 hasRelatedWork W3189034321 @default.
• W2080940688 hasRelatedWork W3192599908 @default.
• W2080940688 hasRelatedWork W3208802168 @default.
• W2080940688 isParatext "false" @default.
• W2080940688 isRetracted "false" @default.
• W2080940688 magId "2080940688" @default.
• W2080940688 workType "article" @default.