Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080971333> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2080971333 endingPage "433" @default.
- W2080971333 startingPage "429" @default.
- W2080971333 abstract "Six monoclonal antibodies (MAbs) were raised in mice against the 26-kDa glutathione S-transferase (GST) of the parasite Schistosoma japonicum. These MAbs were originally selected for their specific binding to the recombinant GST (r-GST) generated in E. coli by an enzyme-linked immunosorbent assay. A further study demonstrated that all these MAbs bound to plate-coated GST affinity-purified from the parasite Schistosoma japonicum. However, in Western blotting analysis only a single monoclonal antibody (MAb Y3D7) yielded positive binding. The binding of MAb Y3D7 on Western blotting was further characterized; specific binding was found on other GST fusion proteins and on the authentic 26-kDa GST but not the 28-kDa GST in the total soluble worm proteins from Schistosoma japonicum. Using protein-A-mediated immunoprecipitation, MAbs Y3D7 and Y5D5 precipitated r-GST while in parallel experiments the remaining MAbs did not generate r-GST precipitation. In an alternative co-precipitation experiment, r-GST was first bound to glutathione (GSH) Sepharose beads and subsequently tested for interaction with the MAbs. In this manner, all MAbs except MAb Y5D5 were co-precipitated with the complexes. Thus, these select MAbs readily reacted with GST although their binding characteristics were different. Because GST has been widely used in the generation of fusion proteins for various purposes and is a potential vaccine candidate in controlling schistosomiasis, these MAbs should prove valuable for their application to molecular biology and parasitology." @default.
- W2080971333 created "2016-06-24" @default.
- W2080971333 creator A5016111606 @default.
- W2080971333 creator A5017252334 @default.
- W2080971333 creator A5032649678 @default.
- W2080971333 creator A5066329762 @default.
- W2080971333 date "1996-12-01" @default.
- W2080971333 modified "2023-09-25" @default.
- W2080971333 title "Characterization of Monoclonal Antibodies to the 26-kDa Glutathione S-transferase of Schistosoma japonicum" @default.
- W2080971333 cites W1869532508 @default.
- W2080971333 cites W1990147618 @default.
- W2080971333 cites W2001101912 @default.
- W2080971333 cites W2002734653 @default.
- W2080971333 cites W2011309130 @default.
- W2080971333 cites W2030017358 @default.
- W2080971333 cites W2040758860 @default.
- W2080971333 cites W2052783086 @default.
- W2080971333 cites W2068446924 @default.
- W2080971333 cites W2091091741 @default.
- W2080971333 cites W2142574026 @default.
- W2080971333 doi "https://doi.org/10.1089/hyb.1996.15.429" @default.
- W2080971333 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8985754" @default.
- W2080971333 hasPublicationYear "1996" @default.
- W2080971333 type Work @default.
- W2080971333 sameAs 2080971333 @default.
- W2080971333 citedByCount "10" @default.
- W2080971333 crossrefType "journal-article" @default.
- W2080971333 hasAuthorship W2080971333A5016111606 @default.
- W2080971333 hasAuthorship W2080971333A5017252334 @default.
- W2080971333 hasAuthorship W2080971333A5032649678 @default.
- W2080971333 hasAuthorship W2080971333A5066329762 @default.
- W2080971333 hasConcept C104317684 @default.
- W2080971333 hasConcept C123894998 @default.
- W2080971333 hasConcept C153911025 @default.
- W2080971333 hasConcept C159654299 @default.
- W2080971333 hasConcept C165901193 @default.
- W2080971333 hasConcept C179247698 @default.
- W2080971333 hasConcept C181199279 @default.
- W2080971333 hasConcept C203014093 @default.
- W2080971333 hasConcept C2776225656 @default.
- W2080971333 hasConcept C2776907368 @default.
- W2080971333 hasConcept C2777123769 @default.
- W2080971333 hasConcept C2779881004 @default.
- W2080971333 hasConcept C40767141 @default.
- W2080971333 hasConcept C538909803 @default.
- W2080971333 hasConcept C542903549 @default.
- W2080971333 hasConcept C55493867 @default.
- W2080971333 hasConcept C71829478 @default.
- W2080971333 hasConcept C85265743 @default.
- W2080971333 hasConcept C86803240 @default.
- W2080971333 hasConceptScore W2080971333C104317684 @default.
- W2080971333 hasConceptScore W2080971333C123894998 @default.
- W2080971333 hasConceptScore W2080971333C153911025 @default.
- W2080971333 hasConceptScore W2080971333C159654299 @default.
- W2080971333 hasConceptScore W2080971333C165901193 @default.
- W2080971333 hasConceptScore W2080971333C179247698 @default.
- W2080971333 hasConceptScore W2080971333C181199279 @default.
- W2080971333 hasConceptScore W2080971333C203014093 @default.
- W2080971333 hasConceptScore W2080971333C2776225656 @default.
- W2080971333 hasConceptScore W2080971333C2776907368 @default.
- W2080971333 hasConceptScore W2080971333C2777123769 @default.
- W2080971333 hasConceptScore W2080971333C2779881004 @default.
- W2080971333 hasConceptScore W2080971333C40767141 @default.
- W2080971333 hasConceptScore W2080971333C538909803 @default.
- W2080971333 hasConceptScore W2080971333C542903549 @default.
- W2080971333 hasConceptScore W2080971333C55493867 @default.
- W2080971333 hasConceptScore W2080971333C71829478 @default.
- W2080971333 hasConceptScore W2080971333C85265743 @default.
- W2080971333 hasConceptScore W2080971333C86803240 @default.
- W2080971333 hasIssue "6" @default.
- W2080971333 hasLocation W20809713331 @default.
- W2080971333 hasLocation W20809713332 @default.
- W2080971333 hasOpenAccess W2080971333 @default.
- W2080971333 hasPrimaryLocation W20809713331 @default.
- W2080971333 hasRelatedWork W2059499224 @default.
- W2080971333 hasRelatedWork W2080971333 @default.
- W2080971333 hasRelatedWork W2105804944 @default.
- W2080971333 hasRelatedWork W2357972350 @default.
- W2080971333 hasRelatedWork W2368460920 @default.
- W2080971333 hasRelatedWork W2372506654 @default.
- W2080971333 hasRelatedWork W2373329635 @default.
- W2080971333 hasRelatedWork W2377988684 @default.
- W2080971333 hasRelatedWork W2565802540 @default.
- W2080971333 hasRelatedWork W3032228465 @default.
- W2080971333 hasVolume "15" @default.
- W2080971333 isParatext "false" @default.
- W2080971333 isRetracted "false" @default.
- W2080971333 magId "2080971333" @default.
- W2080971333 workType "article" @default.