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- W2080971871 abstract "— A membrane-bound form of GP-350, a sialoglycoprotein from calf brain has been shown to occur in the crude mitochondrial fraction (P2). After subfractionation on a discontinuous sucrose gradient, consisting of 0.8 and 1.2 m-sucrose, GP-350 was immunologically only detectable in the synaptosomal fraction. After further separation of the lysed synaptosomal fraction on a discontinuous sucrose gradient (0.4, 0.8 and 1.2m) GP-350 could be detected only in the 0.8–1.2 m-sucrose interface.The membrane-bound GP-350 from the cerebellar grey matter was purified and analysed. The amino acid composition and the data obtained for galactose, galactosamine, glucosamine and sialic acid were quite similar to those of the soluble GP-350, indicating their identity, which was sustained by immunodif-fusion, immunoelectrophoresis and polyacrylamide gel electrophoresis.The membrane-bound GP-350 was isolated from 15 different brain areas. Per g wet wt at least 60 μg (corpora quadrigemina inferior) and at most 470 μg (thalamus) of GP-350 protein were obtained. Compared to the soluble GP-350 protein 10–52 per cent could be isolated from the crude mitochondrial fraction. Our results on GP-350 indicate an association with membranes of nerve endings." @default.
- W2080971871 created "2016-06-24" @default.
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- W2080971871 date "1974-07-01" @default.
- W2080971871 modified "2023-09-25" @default.
- W2080971871 title "GP-350, A SIALOGLYCOPROTEIN FROM CALF BRAIN: ITS SUBCELLULAR LOCALIZATION AND OCCURRENCE IN VARIOUS BRAIN AREAS" @default.
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- W2080971871 doi "https://doi.org/10.1111/j.1471-4159.1974.tb06919.x" @default.
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