Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080976446> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2080976446 endingPage "194" @default.
- W2080976446 startingPage "190" @default.
- W2080976446 abstract "The number and activity of ouabain-sensitive Na/K ATPase pumps expressed by many cell types in vitro, including human retinal pigment epithelial cells (RPE), have been shown to decline with increasing culture density. Cell proliferation also declined as cultures became dense so it was unclear if pump number was modulated by cell proliferation or culture confluency. By exposing RPE cultures to various feeding regimens, using culture medium containing or lacking serum, it was possible to produce RPE cultures with a range of culture densities and growth rates. These were analyzed for proliferative activity by quantifying [3H]thymidine incorporation and for Na/K ATPase pump number by measuring specific [3H]ouabain binding. The results suggest that pump number is modulated by culture density and, further, that the density-dependent regulation of pump number requires serum. Although density-dependent modulation of culture growth is also serum requiring, cell proliferation and pump number did not appear to be related; cultures of similar density which differed significantly in growth rate had similar numbers of pumps. The view that elevated numbers of pumps were not necessarily found in proliferating cells was further supported by qualitative examination of radioautographs of cells dually labeled with [3H]thymidine and [3H]ouabain. Cycling cells which had [3H]thymidine-labeled nuclei did not have notably higher labeling with [3H]ouabain. However, [3H]ouabain labeling, as an indicator of pump site number and distribution, did vary among cells in an RPE population and also within individual cells. This latter observation suggests that unpolarized RPE cells in sparse cultures may have regionally different requirements for ionic regulation." @default.
- W2080976446 created "2016-06-24" @default.
- W2080976446 creator A5059461231 @default.
- W2080976446 creator A5060191984 @default.
- W2080976446 creator A5090864408 @default.
- W2080976446 date "1991-06-01" @default.
- W2080976446 modified "2023-10-18" @default.
- W2080976446 title "The effect of culture density and proliferation rate on the expression of ouabain-sensitive NaK ATPase pumps in cultured human retinal pigment epithelium" @default.
- W2080976446 cites W1575266405 @default.
- W2080976446 cites W1972233556 @default.
- W2080976446 cites W1980518293 @default.
- W2080976446 cites W1992472345 @default.
- W2080976446 cites W2031785542 @default.
- W2080976446 cites W2049959318 @default.
- W2080976446 cites W2050444768 @default.
- W2080976446 cites W2068604627 @default.
- W2080976446 cites W2083243252 @default.
- W2080976446 cites W2089895528 @default.
- W2080976446 cites W2150814613 @default.
- W2080976446 cites W2159930645 @default.
- W2080976446 doi "https://doi.org/10.1016/0014-4827(91)90353-v" @default.
- W2080976446 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1851094" @default.
- W2080976446 hasPublicationYear "1991" @default.
- W2080976446 type Work @default.
- W2080976446 sameAs 2080976446 @default.
- W2080976446 citedByCount "11" @default.
- W2080976446 countsByYear W20809764462017 @default.
- W2080976446 crossrefType "journal-article" @default.
- W2080976446 hasAuthorship W2080976446A5059461231 @default.
- W2080976446 hasAuthorship W2080976446A5060191984 @default.
- W2080976446 hasAuthorship W2080976446A5090864408 @default.
- W2080976446 hasConcept C1491633281 @default.
- W2080976446 hasConcept C153911025 @default.
- W2080976446 hasConcept C178790620 @default.
- W2080976446 hasConcept C181199279 @default.
- W2080976446 hasConcept C185592680 @default.
- W2080976446 hasConcept C202751555 @default.
- W2080976446 hasConcept C23265538 @default.
- W2080976446 hasConcept C2777108182 @default.
- W2080976446 hasConcept C2778024200 @default.
- W2080976446 hasConcept C2779133857 @default.
- W2080976446 hasConcept C2780827179 @default.
- W2080976446 hasConcept C537181965 @default.
- W2080976446 hasConcept C54355233 @default.
- W2080976446 hasConcept C55493867 @default.
- W2080976446 hasConcept C62112901 @default.
- W2080976446 hasConcept C81885089 @default.
- W2080976446 hasConcept C86803240 @default.
- W2080976446 hasConcept C95444343 @default.
- W2080976446 hasConceptScore W2080976446C1491633281 @default.
- W2080976446 hasConceptScore W2080976446C153911025 @default.
- W2080976446 hasConceptScore W2080976446C178790620 @default.
- W2080976446 hasConceptScore W2080976446C181199279 @default.
- W2080976446 hasConceptScore W2080976446C185592680 @default.
- W2080976446 hasConceptScore W2080976446C202751555 @default.
- W2080976446 hasConceptScore W2080976446C23265538 @default.
- W2080976446 hasConceptScore W2080976446C2777108182 @default.
- W2080976446 hasConceptScore W2080976446C2778024200 @default.
- W2080976446 hasConceptScore W2080976446C2779133857 @default.
- W2080976446 hasConceptScore W2080976446C2780827179 @default.
- W2080976446 hasConceptScore W2080976446C537181965 @default.
- W2080976446 hasConceptScore W2080976446C54355233 @default.
- W2080976446 hasConceptScore W2080976446C55493867 @default.
- W2080976446 hasConceptScore W2080976446C62112901 @default.
- W2080976446 hasConceptScore W2080976446C81885089 @default.
- W2080976446 hasConceptScore W2080976446C86803240 @default.
- W2080976446 hasConceptScore W2080976446C95444343 @default.
- W2080976446 hasIssue "2" @default.
- W2080976446 hasLocation W20809764461 @default.
- W2080976446 hasLocation W20809764462 @default.
- W2080976446 hasOpenAccess W2080976446 @default.
- W2080976446 hasPrimaryLocation W20809764461 @default.
- W2080976446 hasRelatedWork W2066519212 @default.
- W2080976446 hasRelatedWork W2083693850 @default.
- W2080976446 hasRelatedWork W2122891300 @default.
- W2080976446 hasRelatedWork W2134199152 @default.
- W2080976446 hasRelatedWork W2363880822 @default.
- W2080976446 hasRelatedWork W2374592133 @default.
- W2080976446 hasRelatedWork W2415544842 @default.
- W2080976446 hasRelatedWork W2999831276 @default.
- W2080976446 hasRelatedWork W4307860292 @default.
- W2080976446 hasRelatedWork W67148313 @default.
- W2080976446 hasVolume "194" @default.
- W2080976446 isParatext "false" @default.
- W2080976446 isRetracted "false" @default.
- W2080976446 magId "2080976446" @default.
- W2080976446 workType "article" @default.