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- W2080984458 abstract "The effect of high pressure and heat treatment of human milk on denaturation of lactoferrin was studied. The degree of protein denaturation was estimated using a sandwich enzyme-linked immunosorbent assay. Kinetic parameters for pressure-induced denaturation of lactoferrin were determined over the pressure range 300–650 MPa at 20 °C. Treatment at 300, 400, 500 and 600 MPa for 15 min denatured 9%, 23%, 34% and 48% of lactoferrin, respectively, whereas treatment at 65 °C for 30 min denatured 80%. Pressure-induced denaturation of lactoferrin followed a reaction order of n = 3. Values for the denaturation rate constant ranged from 1.9 × 104 s−1 at 300 MPa to 58.0 × 104 s−1 at 650 MPa. Heat-induced denaturation of lactoferrin involved protein aggregation by disulphide bonds; covalent interactions did not occur during pressure treatment. Results suggest that high-pressure processing is a potential alternative to thermal pasteurisation, giving greater retention of native lactoferrin." @default.
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- W2080984458 date "2014-12-01" @default.
- W2080984458 modified "2023-10-17" @default.
- W2080984458 title "Kinetic parameters for high-pressure-induced denaturation of lactoferrin in human milk" @default.
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- W2080984458 doi "https://doi.org/10.1016/j.idairyj.2014.07.001" @default.
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