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• W2080985514 abstract "We herein demonstrate that Ni-decorated single-walled carbon nanotube field effect transistors (SWNT-FETs) combined with antibody fragments can be used as effective biosensing platforms. Nanoscales Ni particles 20 to 60 nm in diameter were formed on the sidewalls of SWNT-FETs using an electrochemical method. Carcinoembryonic antigen (CEA)-binding single chain variable fragments (scFvs) with a hexahistidine tag [(his)6] were synthesized using genetic engineering, and ordered immobilization of anti-CEA ScFvs on Ni nanoparticles was achieved by exploiting the specific interaction between hexahistidine and Ni. Whereas randomly oriented anti-CEA scFvs did not impart a noticeable change of conductance upon addition of CEA, a clear increase in conductance was observed using Ni-decorated SWNT-FETs functionalized with engineered scFvs." @default.
• W2080985514 created "2016-06-24" @default.
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• W2080985514 date "2009-10-01" @default.
• W2080985514 modified "2023-10-02" @default.
• W2080985514 title "Oriented Immobilization of Antibody Fragments on Ni-Decorated Single-Walled Carbon Nanotube Devices" @default.
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• W2080985514 doi "https://doi.org/10.1021/nn900540a" @default.
• W2080985514 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19795840" @default.
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