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- W2080991857 abstract "A method is described for purification of staphylococcus and B. anthracis arginases. It consists in the extraction of the crude enzyme by autolysis of the acetone dehydrated bacteria, gel filtration on a Sephadex G-25 column and horizontal zone electrophoresis on Sephadex G-200. Crystallized preparations were obtained by acetone treatment in the cold. The crystal forms of the two bacterial arginases are different. The arginase preparations so obtained are homogeneous in the double diffusion and immunoelectrophoresis in agar gel tests, and also on thin layer gel filtration on Sephadex G-200 superfine and electrophoresis on cellulose acetate strips. The immunological specificity of the two bacterial arginases was shown by double diffusion and immunoelectrophoresis in agar gel." @default.
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- W2080991857 date "1965-01-01" @default.
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- W2080991857 title "Purification of bacterial arginase" @default.
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- W2080991857 doi "https://doi.org/10.1016/s0021-9673(01)97418-7" @default.
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