FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081028576> ?p ?o ?g. }

• W2081028576 endingPage "882" @default.
• W2081028576 startingPage "882" @default.
• W2081028576 abstract "Purpose.: To characterize the properties of connexin 46 hemichannels in differentiating fiber cells isolated from mouse lenses. Methods.: Differentiating fiber cells were isolated from mouse lenses using collagenase. Cellular localization of connexin 50 (Cx50) and connexin 46 (Cx46) was assessed by immunofluorescence. Membrane currents were recorded using whole cell patch clamping. Dye uptake was measured using time-lapse imaging. Results.: In freshly dissociated fiber cells isolated from knockout Cx50 (KOCx50) mouse lenses, removal of external divalent cations induced a macroscopic current composed of large conductance channels. This current was reduced at a holding potential of −60 mV, activated on depolarization, and had a reversal potential near 0 mV. These properties were very similar to those of Cx46 hemichannel currents recorded in single Xenopus oocytes. If the currents observed in divalent cation-free Ringer's solution were due to Cx46 hemichannel opening, then dye influx by gap junctional/hemichannel permeable dyes should be measurable in the fiber cells. To measure dye influx, the authors used the positively charged dyes, propidium iodide (PrI) and 4′-6-diamidino-2-phenylindole (DAPI). In the absence of external calcium, fiber cells took up both dyes. Furthermore, dye influx could be inhibited by hemichannel blockers. To confirm that this current was due to Cx46 hemichannels, the authors studied fiber cells isolated from the lenses of double knockout (Cx46−/−; Cx50−/−) mice and demonstrated that both the calcium-sensitive conductance and dye influx were absent. Conclusions.: These results show that Cx46 can form functional hemichannels in the nonjunctional membrane of fiber cells." @default.
• W2081028576 created "2016-06-24" @default.
• W2081028576 creator A5002476176 @default.
• W2081028576 creator A5031474692 @default.
• W2081028576 creator A5044719208 @default.
• W2081028576 creator A5061229411 @default.
• W2081028576 creator A5082035194 @default.
• W2081028576 date "2011-02-17" @default.
• W2081028576 modified "2023-10-17" @default.
• W2081028576 title "Properties of Connexin 46 Hemichannels in Dissociated Lens Fiber Cells" @default.
• W2081028576 cites W109674622 @default.
• W2081028576 cites W123989659 @default.
• W2081028576 cites W141863921 @default.
• W2081028576 cites W1573567421 @default.
• W2081028576 cites W1964924140 @default.
• W2081028576 cites W1965463380 @default.
• W2081028576 cites W1967949725 @default.
• W2081028576 cites W1968411842 @default.
• W2081028576 cites W1972797509 @default.
• W2081028576 cites W1978204191 @default.
• W2081028576 cites W1982524452 @default.
• W2081028576 cites W1990623062 @default.
• W2081028576 cites W1991829009 @default.
• W2081028576 cites W1995927906 @default.
• W2081028576 cites W2003353368 @default.
• W2081028576 cites W2004617457 @default.
• W2081028576 cites W2005636280 @default.
• W2081028576 cites W2007870366 @default.
• W2081028576 cites W2010853860 @default.
• W2081028576 cites W2012175638 @default.
• W2081028576 cites W2017688904 @default.
• W2081028576 cites W2025106506 @default.
• W2081028576 cites W2032045111 @default.
• W2081028576 cites W2033628989 @default.
• W2081028576 cites W2041525174 @default.
• W2081028576 cites W2041645099 @default.
• W2081028576 cites W2045170222 @default.
• W2081028576 cites W2048297880 @default.
• W2081028576 cites W2049562660 @default.
• W2081028576 cites W2053810563 @default.
• W2081028576 cites W2066140320 @default.
• W2081028576 cites W2072982588 @default.
• W2081028576 cites W2098660246 @default.
• W2081028576 cites W2098712686 @default.
• W2081028576 cites W2109127883 @default.
• W2081028576 cites W2123165969 @default.
• W2081028576 cites W2125511062 @default.
• W2081028576 cites W2138356252 @default.
• W2081028576 cites W2142715166 @default.
• W2081028576 cites W2149822541 @default.
• W2081028576 cites W2161305913 @default.
• W2081028576 cites W2493680686 @default.
• W2081028576 cites W63438539 @default.
• W2081028576 cites W98532952 @default.
• W2081028576 cites W184873337 @default.
• W2081028576 doi "https://doi.org/10.1167/iovs.10-6200" @default.
• W2081028576 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3053112" @default.
• W2081028576 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20861491" @default.
• W2081028576 hasPublicationYear "2011" @default.
• W2081028576 type Work @default.
• W2081028576 sameAs 2081028576 @default.
• W2081028576 citedByCount "42" @default.
• W2081028576 countsByYear W20810285762012 @default.
• W2081028576 countsByYear W20810285762013 @default.
• W2081028576 countsByYear W20810285762014 @default.
• W2081028576 countsByYear W20810285762015 @default.
• W2081028576 countsByYear W20810285762016 @default.
• W2081028576 countsByYear W20810285762017 @default.
• W2081028576 countsByYear W20810285762018 @default.
• W2081028576 countsByYear W20810285762019 @default.
• W2081028576 countsByYear W20810285762020 @default.
• W2081028576 countsByYear W20810285762021 @default.
• W2081028576 countsByYear W20810285762022 @default.
• W2081028576 countsByYear W20810285762023 @default.
• W2081028576 crossrefType "journal-article" @default.
• W2081028576 hasAuthorship W2081028576A5002476176 @default.
• W2081028576 hasAuthorship W2081028576A5031474692 @default.
• W2081028576 hasAuthorship W2081028576A5044719208 @default.
• W2081028576 hasAuthorship W2081028576A5061229411 @default.
• W2081028576 hasAuthorship W2081028576A5082035194 @default.
• W2081028576 hasBestOaLocation W20810285761 @default.
• W2081028576 hasConcept C104317684 @default.
• W2081028576 hasConcept C12554922 @default.
• W2081028576 hasConcept C147944092 @default.
• W2081028576 hasConcept C158157758 @default.
• W2081028576 hasConcept C170493617 @default.
• W2081028576 hasConcept C178790620 @default.
• W2081028576 hasConcept C181911157 @default.
• W2081028576 hasConcept C185592680 @default.
• W2081028576 hasConcept C190283241 @default.
• W2081028576 hasConcept C201685922 @default.
• W2081028576 hasConcept C2775934118 @default.
• W2081028576 hasConcept C2776872141 @default.
• W2081028576 hasConcept C2779535977 @default.
• W2081028576 hasConcept C2780228694 @default.
• W2081028576 hasConcept C31573885 @default.
• W2081028576 hasConcept C4141045 @default.
• W2081028576 hasConcept C41625074 @default.

• next