FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081036272> ?p ?o ?g. }

• W2081036272 endingPage "5591" @default.
• W2081036272 startingPage "5581" @default.
• W2081036272 abstract "ABSTRACT The internal hydrophobic sequence within the flaviviral capsid protein (protein C) plays an important role in the assembly of infectious virions. Here, this sequence was analyzed in a West Nile virus lineage I isolate (crow V76/1). An infectious cDNA clone was constructed and used to introduce deletions into the internal hydrophobic domain which comprises helix α2 and part of the loop intervening helices α2 and α3. In total, nine capsid deletion mutants (4 to 14 amino acids long) were constructed and tested for virus viability. Some of the short deletions did not significantly affect growth in cell culture, whereas larger deletions removing almost the entire hydrophobic region significantly impaired viral growth. Efficient growth of the majority of mutants could, however, be restored by the acquisition of second-site mutations. In most cases, these resuscitating mutations were point mutations within protein C changing individual amino acids into more hydrophobic residues, reminiscent of what had been observed previously for another flavivirus, tick-borne encephalitis virus. However, we also identified viable spontaneous pseudorevertants with more than one-third of the capsid protein removed, i.e., 36 or 37 of a total of 105 residues, including all of helix α3 and a hydrophilic segment connecting α3 and α4. These large deletions are predicted to induce formation of large, predominantly hydrophobic fusion helices which may substitute for the loss of the internal hydrophobic domain, underlining the unrivaled structural and functional flexibility of protein C." @default.
• W2081036272 created "2016-06-24" @default.
• W2081036272 creator A5004047390 @default.
• W2081036272 creator A5004796046 @default.
• W2081036272 creator A5006496188 @default.
• W2081036272 creator A5023166133 @default.
• W2081036272 creator A5023428470 @default.
• W2081036272 creator A5029992958 @default.
• W2081036272 creator A5034853599 @default.
• W2081036272 creator A5044965784 @default.
• W2081036272 creator A5080958982 @default.
• W2081036272 date "2009-06-01" @default.
• W2081036272 modified "2023-09-23" @default.
• W2081036272 title "Helices α2 and α3 of West Nile Virus Capsid Protein Are Dispensable for Assembly of Infectious Virions" @default.
• W2081036272 cites W1517586135 @default.
• W2081036272 cites W1517848055 @default.
• W2081036272 cites W1530508050 @default.
• W2081036272 cites W1599389506 @default.
• W2081036272 cites W1911781239 @default.
• W2081036272 cites W1975304761 @default.
• W2081036272 cites W1978035722 @default.
• W2081036272 cites W1978113142 @default.
• W2081036272 cites W1982090618 @default.
• W2081036272 cites W2002545308 @default.
• W2081036272 cites W2004145987 @default.
• W2081036272 cites W2014366923 @default.
• W2081036272 cites W2019186461 @default.
• W2081036272 cites W2023872420 @default.
• W2081036272 cites W2033218860 @default.
• W2081036272 cites W2039642685 @default.
• W2081036272 cites W2043533590 @default.
• W2081036272 cites W2043969835 @default.
• W2081036272 cites W2045740959 @default.
• W2081036272 cites W2047684538 @default.
• W2081036272 cites W2055247746 @default.
• W2081036272 cites W2062183101 @default.
• W2081036272 cites W2070179005 @default.
• W2081036272 cites W2083009436 @default.
• W2081036272 cites W2087599637 @default.
• W2081036272 cites W2093365802 @default.
• W2081036272 cites W2103438957 @default.
• W2081036272 cites W2104294010 @default.
• W2081036272 cites W2105538224 @default.
• W2081036272 cites W2109402946 @default.
• W2081036272 cites W2118586702 @default.
• W2081036272 cites W2121553667 @default.
• W2081036272 cites W2131968081 @default.
• W2081036272 cites W2133783391 @default.
• W2081036272 cites W2135778915 @default.
• W2081036272 cites W2137769552 @default.
• W2081036272 cites W2140630012 @default.
• W2081036272 cites W2141128138 @default.
• W2081036272 cites W2146321324 @default.
• W2081036272 cites W2149824411 @default.
• W2081036272 cites W2153187042 @default.
• W2081036272 cites W2155379649 @default.
• W2081036272 cites W2163412375 @default.
• W2081036272 cites W2163896334 @default.
• W2081036272 cites W2166505644 @default.
• W2081036272 cites W2289302180 @default.
• W2081036272 cites W4296765338 @default.
• W2081036272 doi "https://doi.org/10.1128/jvi.02653-08" @default.
• W2081036272 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2681950" @default.
• W2081036272 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19297470" @default.
• W2081036272 hasPublicationYear "2009" @default.
• W2081036272 type Work @default.
• W2081036272 sameAs 2081036272 @default.
• W2081036272 citedByCount "32" @default.
• W2081036272 countsByYear W20810362722012 @default.
• W2081036272 countsByYear W20810362722013 @default.
• W2081036272 countsByYear W20810362722014 @default.
• W2081036272 countsByYear W20810362722015 @default.
• W2081036272 countsByYear W20810362722016 @default.
• W2081036272 countsByYear W20810362722018 @default.
• W2081036272 countsByYear W20810362722019 @default.
• W2081036272 countsByYear W20810362722020 @default.
• W2081036272 countsByYear W20810362722021 @default.
• W2081036272 countsByYear W20810362722023 @default.
• W2081036272 crossrefType "journal-article" @default.
• W2081036272 hasAuthorship W2081036272A5004047390 @default.
• W2081036272 hasAuthorship W2081036272A5004796046 @default.
• W2081036272 hasAuthorship W2081036272A5006496188 @default.
• W2081036272 hasAuthorship W2081036272A5023166133 @default.
• W2081036272 hasAuthorship W2081036272A5023428470 @default.
• W2081036272 hasAuthorship W2081036272A5029992958 @default.
• W2081036272 hasAuthorship W2081036272A5034853599 @default.
• W2081036272 hasAuthorship W2081036272A5044965784 @default.
• W2081036272 hasAuthorship W2081036272A5080958982 @default.
• W2081036272 hasBestOaLocation W20810362722 @default.
• W2081036272 hasConcept C104317684 @default.
• W2081036272 hasConcept C143065580 @default.
• W2081036272 hasConcept C144292202 @default.
• W2081036272 hasConcept C153911025 @default.
• W2081036272 hasConcept C159047783 @default.
• W2081036272 hasConcept C167625842 @default.
• W2081036272 hasConcept C176944494 @default.
• W2081036272 hasConcept C202878990 @default.
• W2081036272 hasConcept C2522874641 @default.

• next