FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081142301> ?p ?o ?g. }

• W2081142301 endingPage "7212" @default.
• W2081142301 startingPage "7205" @default.
• W2081142301 abstract "Genetically modified (GM) papaya (Carica papaya L.), Huanong No. 1, was approved for commercialization in Guangdong province, China in 2006, and the development of the Huanong No. 1 papaya detection method is necessary for implementing genetically modified organism (GMO) labeling regulations. In this study, we reported the characterization of the exogenous integration of GM Huanong No. 1 papaya by means of conventional polymerase chain reaction (PCR) and thermal asymmetric interlaced (TAIL)-PCR strategies. The results suggested that one intact copy of the initial construction was integrated in the papaya genome and which probably resulted in one deletion (38 bp in size) of the host genomic DNA. Also, one unintended insertion of a 92 bp truncated NptII fragment was observed at the 5′ end of the exogenous insert. Furthermore, we revealed its 5′ and 3′ flanking sequences between the insert DNA and the papaya genomic DNA, and developed the event-specific qualitative and quantitative PCR assays for GM Huanong No. 1 papaya based on the 5′ integration flanking sequence. The relative limit of detection (LOD) of the qualitative PCR assay was about 0.01% in 100 ng of total papaya genomic DNA, corresponding to about 25 copies of papaya haploid genome. In the quantitative PCR, the limits of detection and quantification (LOD and LOQ) were as low as 12.5 and 25 copies of papaya haploid genome, respectively. In practical sample quantification, the quantified biases between the test and true values of three samples ranged from 0.44% to 4.41%. Collectively, we proposed that all of these results are useful for the identification and quantification of Huanong No. 1 papaya and its derivates." @default.
• W2081142301 created "2016-06-24" @default.
• W2081142301 creator A5002437982 @default.
• W2081142301 creator A5007370787 @default.
• W2081142301 creator A5010816185 @default.
• W2081142301 creator A5035772816 @default.
• W2081142301 creator A5053571254 @default.
• W2081142301 creator A5054018062 @default.
• W2081142301 date "2009-07-31" @default.
• W2081142301 modified "2023-10-07" @default.
• W2081142301 title "Characterization of the Exogenous Insert and Development of Event-specific PCR Detection Methods for Genetically Modified Huanong No. 1 Papaya" @default.
• W2081142301 cites W1597769840 @default.
• W2081142301 cites W2008488883 @default.
• W2081142301 cites W2035443736 @default.
• W2081142301 cites W2037188174 @default.
• W2081142301 cites W2052602128 @default.
• W2081142301 cites W2080859197 @default.
• W2081142301 cites W2084940897 @default.
• W2081142301 cites W2091344480 @default.
• W2081142301 cites W2092147415 @default.
• W2081142301 cites W2093552551 @default.
• W2081142301 cites W2093794734 @default.
• W2081142301 cites W2117546659 @default.
• W2081142301 cites W2121307281 @default.
• W2081142301 cites W2128629908 @default.
• W2081142301 cites W69812398 @default.
• W2081142301 doi "https://doi.org/10.1021/jf901198x" @default.
• W2081142301 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19645503" @default.
• W2081142301 hasPublicationYear "2009" @default.
• W2081142301 type Work @default.
• W2081142301 sameAs 2081142301 @default.
• W2081142301 citedByCount "31" @default.
• W2081142301 countsByYear W20811423012012 @default.
• W2081142301 countsByYear W20811423012013 @default.
• W2081142301 countsByYear W20811423012014 @default.
• W2081142301 countsByYear W20811423012015 @default.
• W2081142301 countsByYear W20811423012016 @default.
• W2081142301 countsByYear W20811423012018 @default.
• W2081142301 countsByYear W20811423012019 @default.
• W2081142301 countsByYear W20811423012020 @default.
• W2081142301 countsByYear W20811423012021 @default.
• W2081142301 countsByYear W20811423012022 @default.
• W2081142301 countsByYear W20811423012023 @default.
• W2081142301 crossrefType "journal-article" @default.
• W2081142301 hasAuthorship W2081142301A5002437982 @default.
• W2081142301 hasAuthorship W2081142301A5007370787 @default.
• W2081142301 hasAuthorship W2081142301A5010816185 @default.
• W2081142301 hasAuthorship W2081142301A5035772816 @default.
• W2081142301 hasAuthorship W2081142301A5053571254 @default.
• W2081142301 hasAuthorship W2081142301A5054018062 @default.
• W2081142301 hasConcept C104317684 @default.
• W2081142301 hasConcept C127413603 @default.
• W2081142301 hasConcept C141231307 @default.
• W2081142301 hasConcept C153911025 @default.
• W2081142301 hasConcept C17757408 @default.
• W2081142301 hasConcept C2779231358 @default.
• W2081142301 hasConcept C2780098074 @default.
• W2081142301 hasConcept C48023723 @default.
• W2081142301 hasConcept C49105822 @default.
• W2081142301 hasConcept C51456166 @default.
• W2081142301 hasConcept C54355233 @default.
• W2081142301 hasConcept C552990157 @default.
• W2081142301 hasConcept C59822182 @default.
• W2081142301 hasConcept C78519656 @default.
• W2081142301 hasConcept C86803240 @default.
• W2081142301 hasConceptScore W2081142301C104317684 @default.
• W2081142301 hasConceptScore W2081142301C127413603 @default.
• W2081142301 hasConceptScore W2081142301C141231307 @default.
• W2081142301 hasConceptScore W2081142301C153911025 @default.
• W2081142301 hasConceptScore W2081142301C17757408 @default.
• W2081142301 hasConceptScore W2081142301C2779231358 @default.
• W2081142301 hasConceptScore W2081142301C2780098074 @default.
• W2081142301 hasConceptScore W2081142301C48023723 @default.
• W2081142301 hasConceptScore W2081142301C49105822 @default.
• W2081142301 hasConceptScore W2081142301C51456166 @default.
• W2081142301 hasConceptScore W2081142301C54355233 @default.
• W2081142301 hasConceptScore W2081142301C552990157 @default.
• W2081142301 hasConceptScore W2081142301C59822182 @default.
• W2081142301 hasConceptScore W2081142301C78519656 @default.
• W2081142301 hasConceptScore W2081142301C86803240 @default.
• W2081142301 hasIssue "16" @default.
• W2081142301 hasLocation W20811423011 @default.
• W2081142301 hasLocation W20811423012 @default.
• W2081142301 hasOpenAccess W2081142301 @default.
• W2081142301 hasPrimaryLocation W20811423011 @default.
• W2081142301 hasRelatedWork W1930314440 @default.
• W2081142301 hasRelatedWork W2347895174 @default.
• W2081142301 hasRelatedWork W2357385872 @default.
• W2081142301 hasRelatedWork W2374544627 @default.
• W2081142301 hasRelatedWork W2381593134 @default.
• W2081142301 hasRelatedWork W2469595084 @default.
• W2081142301 hasRelatedWork W2520606592 @default.
• W2081142301 hasRelatedWork W2737874409 @default.
• W2081142301 hasRelatedWork W2803086839 @default.
• W2081142301 hasRelatedWork W4236824791 @default.
• W2081142301 hasVolume "57" @default.
• W2081142301 isParatext "false" @default.
• W2081142301 isRetracted "false" @default.

• next