FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081150812> ?p ?o ?g. }

• W2081150812 endingPage "6148" @default.
• W2081150812 startingPage "6140" @default.
• W2081150812 abstract "Rational redesign of the binding pocket of Cellular Retinoic Acid Binding Protein II (CRABPII) has provided a mutant that can bind retinal as a protonated Schiff base, mimicking the binding observed in rhodopsin. The reengineering was accomplished through a series of choreographed manipulations to ultimately orient the reactive species (the ε-amino group of Lys132 and the carbonyl of retinal) in the proper geometry for imine formation. The guiding principle was to achieve the appropriate Bürgi−Dunitz trajectory for the reaction to ensue. Through crystallographic analysis of protein mutants incapable of forming the requisite Schiff base, a highly ordered water molecule was identified as a key culprit in orienting retinal in a nonconstructive manner. Removal of the ordered water, along with placing reinforcing mutations to favor the desired orientation of retinal, led to a triple mutant CRABPII protein capable of nanomolar binding of retinal as a protonated Schiff base. The high-resolution crystal structure of all-trans-retinal bound to the CRABPII triple mutant (1.2 Å resolution) unequivocally illustrates the imine formed between retinal and the protein." @default.
• W2081150812 created "2016-06-24" @default.
• W2081150812 creator A5001811134 @default.
• W2081150812 creator A5004761952 @default.
• W2081150812 creator A5037649573 @default.
• W2081150812 creator A5051291962 @default.
• W2081150812 creator A5052952898 @default.
• W2081150812 creator A5068625144 @default.
• W2081150812 date "2007-04-21" @default.
• W2081150812 modified "2023-10-17" @default.
• W2081150812 title "Protein Design: Reengineering Cellular Retinoic Acid Binding Protein II into a Rhodopsin Protein Mimic" @default.
• W2081150812 cites W106664804 @default.
• W2081150812 cites W11595199 @default.
• W2081150812 cites W1487590544 @default.
• W2081150812 cites W1536665845 @default.
• W2081150812 cites W1590004953 @default.
• W2081150812 cites W1963480398 @default.
• W2081150812 cites W1965205049 @default.
• W2081150812 cites W1965664542 @default.
• W2081150812 cites W1967125817 @default.
• W2081150812 cites W1967779980 @default.
• W2081150812 cites W1978248125 @default.
• W2081150812 cites W1979212500 @default.
• W2081150812 cites W1980149636 @default.
• W2081150812 cites W1981152907 @default.
• W2081150812 cites W1989988432 @default.
• W2081150812 cites W1992061014 @default.
• W2081150812 cites W1992636466 @default.
• W2081150812 cites W1997220351 @default.
• W2081150812 cites W1998120428 @default.
• W2081150812 cites W1999116701 @default.
• W2081150812 cites W2001446706 @default.
• W2081150812 cites W2006043898 @default.
• W2081150812 cites W2007712861 @default.
• W2081150812 cites W2014059004 @default.
• W2081150812 cites W2015875673 @default.
• W2081150812 cites W2017272071 @default.
• W2081150812 cites W2019053303 @default.
• W2081150812 cites W2032196002 @default.
• W2081150812 cites W2040321848 @default.
• W2081150812 cites W2041136899 @default.
• W2081150812 cites W2049486769 @default.
• W2081150812 cites W2049852872 @default.
• W2081150812 cites W2062540699 @default.
• W2081150812 cites W2062661644 @default.
• W2081150812 cites W2066510935 @default.
• W2081150812 cites W2067236472 @default.
• W2081150812 cites W2072144082 @default.
• W2081150812 cites W2073274300 @default.
• W2081150812 cites W2076023906 @default.
• W2081150812 cites W2077228933 @default.
• W2081150812 cites W2081368534 @default.
• W2081150812 cites W2084535723 @default.
• W2081150812 cites W2085497102 @default.
• W2081150812 cites W2086404667 @default.
• W2081150812 cites W2090297991 @default.
• W2081150812 cites W2115357061 @default.
• W2081150812 cites W2117026594 @default.
• W2081150812 cites W2124804840 @default.
• W2081150812 cites W2127351444 @default.
• W2081150812 cites W2131173272 @default.
• W2081150812 cites W2137588110 @default.
• W2081150812 cites W2138817605 @default.
• W2081150812 cites W2145441838 @default.
• W2081150812 cites W2148162804 @default.
• W2081150812 cites W2149963584 @default.
• W2081150812 cites W2151785803 @default.
• W2081150812 cites W2160398112 @default.
• W2081150812 cites W2172049330 @default.
• W2081150812 cites W2951102041 @default.
• W2081150812 cites W4243296320 @default.
• W2081150812 cites W4243838097 @default.
• W2081150812 doi "https://doi.org/10.1021/ja067546r" @default.
• W2081150812 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17447762" @default.
• W2081150812 hasPublicationYear "2007" @default.
• W2081150812 type Work @default.
• W2081150812 sameAs 2081150812 @default.
• W2081150812 citedByCount "47" @default.
• W2081150812 countsByYear W20811508122012 @default.
• W2081150812 countsByYear W20811508122013 @default.
• W2081150812 countsByYear W20811508122014 @default.
• W2081150812 countsByYear W20811508122015 @default.
• W2081150812 countsByYear W20811508122016 @default.
• W2081150812 countsByYear W20811508122017 @default.
• W2081150812 countsByYear W20811508122018 @default.
• W2081150812 countsByYear W20811508122019 @default.
• W2081150812 countsByYear W20811508122020 @default.
• W2081150812 countsByYear W20811508122021 @default.
• W2081150812 crossrefType "journal-article" @default.
• W2081150812 hasAuthorship W2081150812A5001811134 @default.
• W2081150812 hasAuthorship W2081150812A5004761952 @default.
• W2081150812 hasAuthorship W2081150812A5037649573 @default.
• W2081150812 hasAuthorship W2081150812A5051291962 @default.
• W2081150812 hasAuthorship W2081150812A5052952898 @default.
• W2081150812 hasAuthorship W2081150812A5068625144 @default.
• W2081150812 hasConcept C104317684 @default.
• W2081150812 hasConcept C12554922 @default.
• W2081150812 hasConcept C143065580 @default.

• next