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• W2081224842 abstract "Currently, to treat skin defects with artificial dermis (AD), two surgical procedures where the artificial dermis grafting and another secondary skin grafting are required. The purpose of this study was to achieve simultaneous grafting of the artificial dermis and the split-skin. To enhance the wound angiogenesis, cultured endothelial cells, fibroblasts and PDWHF (platelet derived wound healing factor) were employed. The experiment consists of following two parts: (1) Investigation to obtain faster angiogenesis into the bilayer artificial dermis: full-thickness wounds created on the back of the rats were treated with the artificial dermis (Terudermis®, with silicone sheet, TERUMO Co., Japan). Prior to the artificial dermis grafting, following four groups were established; control group (AD alone, n = 6), PDWHF group (AD treated with PDWHF, n = 6), cultured cells group (AD treated with cultured endothelial cells and fibroblasts, n = 6), combination group (AD treated with PDWHF and cultured cells, n = 6). (2) Trial of one-stage grafting of the AD and the skin: simultaneous grafting of the artificial dermis and skin was performed using the same rat model. Before making skin defects, split thickness skin were harvested. Then the skin grafting was carried out immediately after the AD grafting. To allow grafting of the skin onto the artificial dermis, the AD without silicone sheet (Terudermis® without silicone sheet, TERUMO Co., Japan) were used. Two groups, control group (AD alone, n = 3) and treatment group (AD with PDWF and cultures, n = 3) were established. (1) When the artificial dermis were treated with PDWHF, cultured endothelial cells and fibroblasts, vascular invasion into the artificial dermis was observed 5 days after the surgery. (2) In the treatment group, the skin grafted immediately after the artificial dermis grafting was completely taken. The present study revealed that treatment with PDWHF, combined with cultured endothelial cells and fibroblasts, accelerated wound angiogenesis. By this method, one-step grafting procedure of the artificial dermis and the skin is possible." @default.
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• W2081224842 date "2006-05-01" @default.
• W2081224842 modified "2023-10-12" @default.
• W2081224842 title "Novel application method of artificial dermis: One-step grafting procedure of artificial dermis and skin, rat experimental study" @default.
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• W2081224842 doi "https://doi.org/10.1016/j.burns.2005.10.013" @default.
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