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- W2081244653 abstract "We report on the development of a simple technique for obtaining time-domain information using dc detection of fluorescence. We show that this is feasible for assays where a change in lifetime of an indicator occurs in reaction to an analyte, in fluorescence resonance energy transfer for example, and could be particularly useful for assays performed in the scaled-down environment of a lab-on-a-chip. A rate equation model is presented which allows an objective analysis of the relative importance of the key measurement parameters: optical saturation of the fluorophore and excitation pulse characteristics. We present a comparison of the model with a cuvette based analysis of a carbocyanine dye where the excitation source is a 650 nm wavelength, self-pulsing AlGaInP laser diode." @default.
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- W2081244653 date "2005-06-30" @default.
- W2081244653 modified "2023-09-23" @default.
- W2081244653 title "Time-resolved fluorescence measurements using stroboscopic excitation" @default.
- W2081244653 doi "https://doi.org/10.1117/12.632914" @default.
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