FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081248371> ?p ?o ?g. }

• W2081248371 endingPage "A109" @default.
• W2081248371 startingPage "A109.1" @default.
• W2081248371 abstract "<h3>Objectives</h3> We aimed to investigate whether over-expression of miR-210, an oxide-related miR, could protect mesenchymal stromal cells (MSCs) against apoptosis induced by oxidative stress, and if so, what the potential mechanisms involved. <h3>Methods</h3> MSCs, derived from male SD rats, with forced expression of miR-210 or miR-null by lentivirus infection was incubated with or without Peroxide hydrogen (H₂O₂). The reactive oxygen species (ROS) accumulation in MSCs was detected by immunofluoresence probes; cellular survival was measured by MTT assay, Hochest staining and TUNEL examination; superoxidedismutase (SOD) activity and adecreaseinmalonaldehyde (MDA) content were analysed by ELISA assay; and the activation of c-Met pathway was quantified by immunoblot. After application of Crizotinib, an inhibitor specific to c-Met pathway, the above detections were investigated to clarify the mechanisms involved. In an <i>in vivo</i> experiment, the expression of sry gene was checked by quantitative PCR to compare the survival of MSCs-miR-210 and MSCs-miR-null in the infarcted myocardium of female rats on day 4 after transplantation; afterwards, echocardiology was applied to assess the cardiac remodelling and myocardial function in a 4-week follow-up. <h3>Results</h3> It was revealed that forced expression of miR-210 significantly decreased the apoptosis of MSCs against H₂O₂compared with that of miR-null, indicated as follows: a notable decreases in ROS generation (2.34 ± 0.62 vs. 3.98 ± 0.57, <i>P</i><0.05) and apoptotic indices [(15.3 ± 3.87)% vs. (33.8 ± 2.67)%, <i>P</i><0.05]; a dramatic increase in cell viability (2.98 ± 0.67 vs. 1.00 ± 0.74, <i>P</i><0.05), SOD activity (3.91 ± 0.28 vs. 1.00 ± 0.38, <i>P</i><0.05) and MDA content (2.95 ± 0.41 vs. 1.00 ± 0.36, <i>P</i><0.05), which was companied by a remarkable activation of c-Met pathway (2.87 ± 0.34 vs. 1.00 ± 0.52, <i>P</i><0.05). Most importantly, each of the above beneficial effects was attenuated by Crizotinib (<i>P</i> all <0.05). Furthermore, transplantation of MSCs-miR-210 resulted in higher cell survival rates (2.86 ± 0.33 vs. 1.00 ± 0.37, <i>P</i><0.05), therefore significantly improved cardiac remodelling [LVIDd: (5.38 ± 0.61) mm vs. (6.39 ± 0.67) mm, <i>P</i><0.05; LVIDs: (4.92 ± 0.52) mm vs. (5.89 ± 0.51) mm, <i>P</i><0.05;] and statistically ameliorated myocardial function [LVEF: (62.3 ± 5.32)% vs. (42.6 ± 4.38)%, <i>P</i><0.05] than that of MSCs-miR-null. <h3>Conclusions</h3> MiR-210 over-expression may improve MSCs survival in an oxidative stress circumstances through anti-oxidation and c-Met pathways activation, thus enhances their therapeutic effect on myocardial infarction, suggesting that miR-210 was a potential intervention target in the cell-based treatment." @default.
• W2081248371 created "2016-06-24" @default.
• W2081248371 creator A5001624794 @default.
• W2081248371 creator A5009753012 @default.
• W2081248371 creator A5028451769 @default.
• W2081248371 creator A5034776291 @default.
• W2081248371 creator A5043401449 @default.
• W2081248371 creator A5050741650 @default.
• W2081248371 creator A5053743858 @default.
• W2081248371 creator A5055657571 @default.
• W2081248371 creator A5078164215 @default.
• W2081248371 date "2013-08-01" @default.
• W2081248371 modified "2023-09-27" @default.
• W2081248371 title "GW24-e3739 MiR-210 Over-expression Enhances Mesenchymal Stromal Cells Survival in an Oxidative Stress Circumstance though Anti-oxidation and c-Met Pathways Activation" @default.
• W2081248371 doi "https://doi.org/10.1136/heartjnl-2013-304613.297" @default.
• W2081248371 hasPublicationYear "2013" @default.
• W2081248371 type Work @default.
• W2081248371 sameAs 2081248371 @default.
• W2081248371 citedByCount "0" @default.
• W2081248371 crossrefType "journal-article" @default.
• W2081248371 hasAuthorship W2081248371A5001624794 @default.
• W2081248371 hasAuthorship W2081248371A5009753012 @default.
• W2081248371 hasAuthorship W2081248371A5028451769 @default.
• W2081248371 hasAuthorship W2081248371A5034776291 @default.
• W2081248371 hasAuthorship W2081248371A5043401449 @default.
• W2081248371 hasAuthorship W2081248371A5050741650 @default.
• W2081248371 hasAuthorship W2081248371A5053743858 @default.
• W2081248371 hasAuthorship W2081248371A5055657571 @default.
• W2081248371 hasAuthorship W2081248371A5078164215 @default.
• W2081248371 hasConcept C126322002 @default.
• W2081248371 hasConcept C142724271 @default.
• W2081248371 hasConcept C150903083 @default.
• W2081248371 hasConcept C153911025 @default.
• W2081248371 hasConcept C16685009 @default.
• W2081248371 hasConcept C190283241 @default.
• W2081248371 hasConcept C196795494 @default.
• W2081248371 hasConcept C198826908 @default.
• W2081248371 hasConcept C204232928 @default.
• W2081248371 hasConcept C207001950 @default.
• W2081248371 hasConcept C2776151105 @default.
• W2081248371 hasConcept C2780783641 @default.
• W2081248371 hasConcept C48349386 @default.
• W2081248371 hasConcept C55493867 @default.
• W2081248371 hasConcept C71924100 @default.
• W2081248371 hasConcept C86803240 @default.
• W2081248371 hasConcept C95444343 @default.
• W2081248371 hasConceptScore W2081248371C126322002 @default.
• W2081248371 hasConceptScore W2081248371C142724271 @default.
• W2081248371 hasConceptScore W2081248371C150903083 @default.
• W2081248371 hasConceptScore W2081248371C153911025 @default.
• W2081248371 hasConceptScore W2081248371C16685009 @default.
• W2081248371 hasConceptScore W2081248371C190283241 @default.
• W2081248371 hasConceptScore W2081248371C196795494 @default.
• W2081248371 hasConceptScore W2081248371C198826908 @default.
• W2081248371 hasConceptScore W2081248371C204232928 @default.
• W2081248371 hasConceptScore W2081248371C207001950 @default.
• W2081248371 hasConceptScore W2081248371C2776151105 @default.
• W2081248371 hasConceptScore W2081248371C2780783641 @default.
• W2081248371 hasConceptScore W2081248371C48349386 @default.
• W2081248371 hasConceptScore W2081248371C55493867 @default.
• W2081248371 hasConceptScore W2081248371C71924100 @default.
• W2081248371 hasConceptScore W2081248371C86803240 @default.
• W2081248371 hasConceptScore W2081248371C95444343 @default.
• W2081248371 hasIssue "Suppl 3" @default.
• W2081248371 hasLocation W20812483711 @default.
• W2081248371 hasOpenAccess W2081248371 @default.
• W2081248371 hasPrimaryLocation W20812483711 @default.
• W2081248371 hasRelatedWork W2023082526 @default.
• W2081248371 hasRelatedWork W2061260890 @default.
• W2081248371 hasRelatedWork W2080167910 @default.
• W2081248371 hasRelatedWork W2252641125 @default.
• W2081248371 hasRelatedWork W2347577273 @default.
• W2081248371 hasRelatedWork W2362948099 @default.
• W2081248371 hasRelatedWork W2366761680 @default.
• W2081248371 hasRelatedWork W2367700600 @default.
• W2081248371 hasRelatedWork W2621169341 @default.
• W2081248371 hasRelatedWork W4307179377 @default.
• W2081248371 hasVolume "99" @default.
• W2081248371 isParatext "false" @default.
• W2081248371 isRetracted "false" @default.
• W2081248371 magId "2081248371" @default.
• W2081248371 workType "article" @default.