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- W2081256905 abstract "This chapter discusses the analytical techniques used in the chemical speciation of trace elements in biomedical specimens and the major considerations in the choice of techniques applied to the study of the various chemical forms of an element are reviewed. The rates and extents of trace element dependent metabolic reactions are controlled by the activities of the relevant element-containing species. It is necessary to identify and quantify all of these species to understand at the molecular level the roles of these elements in human metabolism. Trace metals interact with proteins, nucleic acids, amino acids, lipids and other ligands to form complexes of varying degrees of thermodynamic stability and reactivity. The factors that affect the stability of metal-ligand interactions such as temperature or ionic strength are described. Some of the numerous fraction techniques that are used for biochemical separation are examined with particular reference to modification that could occur to the distribution of trace element-containing species. The techniques emphasized in the chapter are liquid chromatography, size exclusion chromatography, ion exchange chromatography, and affinity chromatography." @default.
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- W2081256905 date "1968-06-01" @default.
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- W2081256905 title "Precipitation of human serum proteins by polyethyleneglycol" @default.
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- W2081256905 doi "https://doi.org/10.1016/0009-8981(68)90313-6" @default.
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