Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081282051> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2081282051 endingPage "205" @default.
- W2081282051 startingPage "197" @default.
- W2081282051 abstract "The activity of ornithine decarboxylase has been detected for the first time in extracts of a thermophilic bacterium, Clostridium thermohydrosulfuricum. The temperature optimum of the thermoresistant ornithine decarboxylase was 55 degrees C and the pH optimum was 7.5. It required pyridoxal phosphate and a thiol (dithiothreitol) for activity. The activity of the enzyme was closely connected to the growth of the thermophilic bacteria, since the activity was highest during the logarithmic growth. The enzyme was not inhibited (in contrast to the enzyme from Escherichia coli) by putrescine, spermidine or other naturally occurring polyamines. When the effect of GTP and a number of GTP analogues was tested on the activity of the enzyme, it was observed that GTP or dGTP was necessary for the full activity. The modification of either the purine base or 5'-phosphate chain of GTP leads to a stimulation smaller than that caused by GTP. Modification of the 3'-carbon of the ribose part of GTP (magic spot I and II of Cashel and Gallant, Nature 221 (1969) 838-841) caused a distinct inhibition of the enzyme activity, indicating that ornithine decarboxylase contains at least two domains for binding of GTP. The enzyme was inhibited irreversibly by high concentrations (50 mM) of difluoromethylornithine. Extracts of the bacterium contained also arginine decarboxylase, but its activity was always very much lower than that of ornithine decarboxylase. The activity of arginine decarboxylase was inhibited irreversibly by difluoromethylarginine, which is an irreversible suicide inhibitor of bacterial arginine decarboxylase (Kallio, A., McCann, P.P. and Bey, P. (1981) Biochemistry 20, 3163-3166)." @default.
- W2081282051 created "2016-06-24" @default.
- W2081282051 creator A5024411067 @default.
- W2081282051 creator A5048842264 @default.
- W2081282051 date "1983-01-01" @default.
- W2081282051 modified "2023-09-27" @default.
- W2081282051 title "Ornithine decarboxylase activity from an extremely thermophilic bacterium, Clostridium thermohydrosulfuricum effect of GTP analogues on enzyme activity" @default.
- W2081282051 cites W1521287392 @default.
- W2081282051 cites W1541698544 @default.
- W2081282051 cites W1553727277 @default.
- W2081282051 cites W1560631485 @default.
- W2081282051 cites W1775749144 @default.
- W2081282051 cites W1972156120 @default.
- W2081282051 cites W1994749318 @default.
- W2081282051 cites W2012432324 @default.
- W2081282051 cites W2017016334 @default.
- W2081282051 cites W2036062238 @default.
- W2081282051 cites W2040901336 @default.
- W2081282051 cites W2045553639 @default.
- W2081282051 cites W2056797610 @default.
- W2081282051 cites W2059422006 @default.
- W2081282051 cites W2077234133 @default.
- W2081282051 cites W2077629838 @default.
- W2081282051 cites W2080231157 @default.
- W2081282051 cites W2081129124 @default.
- W2081282051 cites W2083991189 @default.
- W2081282051 cites W2095594022 @default.
- W2081282051 cites W346296608 @default.
- W2081282051 cites W35143371 @default.
- W2081282051 doi "https://doi.org/10.1016/0167-4838(83)90377-1" @default.
- W2081282051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6824682" @default.
- W2081282051 hasPublicationYear "1983" @default.
- W2081282051 type Work @default.
- W2081282051 sameAs 2081282051 @default.
- W2081282051 citedByCount "18" @default.
- W2081282051 countsByYear W20812820512014 @default.
- W2081282051 crossrefType "journal-article" @default.
- W2081282051 hasAuthorship W2081282051A5024411067 @default.
- W2081282051 hasAuthorship W2081282051A5048842264 @default.
- W2081282051 hasConcept C108905728 @default.
- W2081282051 hasConcept C118716 @default.
- W2081282051 hasConcept C121289238 @default.
- W2081282051 hasConcept C181199279 @default.
- W2081282051 hasConcept C185592680 @default.
- W2081282051 hasConcept C192233252 @default.
- W2081282051 hasConcept C2776796294 @default.
- W2081282051 hasConcept C2777167505 @default.
- W2081282051 hasConcept C2777468819 @default.
- W2081282051 hasConcept C2777497464 @default.
- W2081282051 hasConcept C2778460671 @default.
- W2081282051 hasConcept C27911776 @default.
- W2081282051 hasConcept C28212737 @default.
- W2081282051 hasConcept C515207424 @default.
- W2081282051 hasConcept C55493867 @default.
- W2081282051 hasConcept C86803240 @default.
- W2081282051 hasConceptScore W2081282051C108905728 @default.
- W2081282051 hasConceptScore W2081282051C118716 @default.
- W2081282051 hasConceptScore W2081282051C121289238 @default.
- W2081282051 hasConceptScore W2081282051C181199279 @default.
- W2081282051 hasConceptScore W2081282051C185592680 @default.
- W2081282051 hasConceptScore W2081282051C192233252 @default.
- W2081282051 hasConceptScore W2081282051C2776796294 @default.
- W2081282051 hasConceptScore W2081282051C2777167505 @default.
- W2081282051 hasConceptScore W2081282051C2777468819 @default.
- W2081282051 hasConceptScore W2081282051C2777497464 @default.
- W2081282051 hasConceptScore W2081282051C2778460671 @default.
- W2081282051 hasConceptScore W2081282051C27911776 @default.
- W2081282051 hasConceptScore W2081282051C28212737 @default.
- W2081282051 hasConceptScore W2081282051C515207424 @default.
- W2081282051 hasConceptScore W2081282051C55493867 @default.
- W2081282051 hasConceptScore W2081282051C86803240 @default.
- W2081282051 hasIssue "1" @default.
- W2081282051 hasLocation W20812820511 @default.
- W2081282051 hasLocation W20812820512 @default.
- W2081282051 hasOpenAccess W2081282051 @default.
- W2081282051 hasPrimaryLocation W20812820511 @default.
- W2081282051 hasRelatedWork W1544175285 @default.
- W2081282051 hasRelatedWork W1554810227 @default.
- W2081282051 hasRelatedWork W1590454468 @default.
- W2081282051 hasRelatedWork W1968714063 @default.
- W2081282051 hasRelatedWork W1991929655 @default.
- W2081282051 hasRelatedWork W2031015954 @default.
- W2081282051 hasRelatedWork W2081282051 @default.
- W2081282051 hasRelatedWork W2091413967 @default.
- W2081282051 hasRelatedWork W2345067921 @default.
- W2081282051 hasRelatedWork W2408067028 @default.
- W2081282051 hasVolume "742" @default.
- W2081282051 isParatext "false" @default.
- W2081282051 isRetracted "false" @default.
- W2081282051 magId "2081282051" @default.
- W2081282051 workType "article" @default.