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• W2081287615 abstract "The use of whole cell biotransformations for single and multistep enzyme conversions is gaining widespread application. In this study the naphthalene dioxygenase nah A gene was transferred into Pseudomonas aeruginosa PAC 1R, Escherichia coli JM107 and Pseudomonas putida PpG 277. The effect of ethanol on these genetically engineered Gram-negative bacteria was studied by measurement of enzyme activity, stability and cell integrity. Ethanol has been used in biotransformations as a co-substrate carbon source for co-factor recycling and as a co-solvent increasing dissolved substrate and product levels. Ethanol increased the dissolved substrate (naphthalene) concentration slightly and dissolved product ((+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene) by approximately 30% at 4% (w/v) ethanol. Both P. aeruginosa PAC 1R and P. putida PpG 277 showed decreased activity with increasing ethanol concentration whilst E. coli enzyme activity increased with increasing ethanol concentration being comparable to that when glucose was used as a carbon source. This project highlighted the many factors involved in the selection of microbial hosts for whole cell biotransformation processes." @default.
• W2081287615 created "2016-06-24" @default.
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• W2081287615 creator A5036903025 @default.
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• W2081287615 date "1996-05-01" @default.
• W2081287615 modified "2023-09-24" @default.
• W2081287615 title "Choice of microbial host for the naphthalene dioxygenase bioconversion" @default.
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• W2081287615 doi "https://doi.org/10.1007/bf01570034" @default.
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• W2081287615 hasPublicationYear "1996" @default.
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