FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081367861> ?p ?o ?g. }

• W2081367861 endingPage "15296" @default.
• W2081367861 startingPage "15280" @default.
• W2081367861 abstract "The tumor invasive phenotype driven by seprase expression/activity has been widely examined in an array of malignant tumor cell types; however, very little is known about the transcriptional regulation of this critical protease. Seprase (also named fibroblast activation protein-α, antiplasmin-cleaving enzyme, and dipeptidyl prolyl peptidase 5) is expressed at high levels by stromal fibroblast, endothelial, and tumor cells in a variety of invasive tumors but is undetectable in the majority of normal adult tissues. To examine the transcriptional regulation of the gene, we cloned the human seprase promoter and demonstrated that endogenous seprase expression and exogenous seprase promoter activity are high in invasive melanoma cells but not in non-invasive melanoma cells/primary melanocytes. In addition, we identified a crucial TGF-β-responsive cis-regulatory element in the proximal seprase promoter region that enabled robust transcriptional activation of the gene. Treatment of metastatic but not normal/non-invasive cells with TGF-β1 caused a rapid and profound up-regulation of endogenous seprase mRNA, which coincided with an abolishment of the negative regulator c-Ski, and an increase in binding of Smad3/4 to the seprase promoter in vivo. Blocking TGF-β signaling in invasive melanoma cells through overexpression of c-Ski, chemically using SB-431542, or with a neutralizing antibody against TGF-β significantly reduced seprase mRNA levels. Strikingly, RNAi of seprase in invasive cells greatly diminished their invasive potential in vitro as did blocking TGF-β signaling using SB-431542. Altogether, we found that seprase is transcriptionally up-regulated in invasive melanoma cells via the canonical TGF-β signaling pathway, supporting the roles of both TGF-β and seprase in tumor invasion and metastasis." @default.
• W2081367861 created "2016-06-24" @default.
• W2081367861 creator A5010483841 @default.
• W2081367861 creator A5087042183 @default.
• W2081367861 date "2014-05-01" @default.
• W2081367861 modified "2023-10-15" @default.
• W2081367861 title "Transcriptional Regulation of Seprase in Invasive Melanoma Cells by Transforming Growth Factor-β Signaling" @default.
• W2081367861 cites W1497682757 @default.
• W2081367861 cites W1565561072 @default.
• W2081367861 cites W1806266807 @default.
• W2081367861 cites W1830180739 @default.
• W2081367861 cites W1964761894 @default.
• W2081367861 cites W1964818072 @default.
• W2081367861 cites W1969683835 @default.
• W2081367861 cites W1975987306 @default.
• W2081367861 cites W1976564364 @default.
• W2081367861 cites W1977017266 @default.
• W2081367861 cites W1984146357 @default.
• W2081367861 cites W1984347910 @default.
• W2081367861 cites W1985412310 @default.
• W2081367861 cites W1985649889 @default.
• W2081367861 cites W1988019564 @default.
• W2081367861 cites W1992592944 @default.
• W2081367861 cites W1994913353 @default.
• W2081367861 cites W2002145465 @default.
• W2081367861 cites W2006351633 @default.
• W2081367861 cites W2014214643 @default.
• W2081367861 cites W2015270653 @default.
• W2081367861 cites W2015441766 @default.
• W2081367861 cites W2026428302 @default.
• W2081367861 cites W2027847535 @default.
• W2081367861 cites W2032600733 @default.
• W2081367861 cites W2047764904 @default.
• W2081367861 cites W2053006673 @default.
• W2081367861 cites W2057043051 @default.
• W2081367861 cites W2061135361 @default.
• W2081367861 cites W2062459814 @default.
• W2081367861 cites W2067582369 @default.
• W2081367861 cites W2072061914 @default.
• W2081367861 cites W2072134991 @default.
• W2081367861 cites W2076541697 @default.
• W2081367861 cites W2080444640 @default.
• W2081367861 cites W2081476887 @default.
• W2081367861 cites W2082050214 @default.
• W2081367861 cites W2085525298 @default.
• W2081367861 cites W2095605427 @default.
• W2081367861 cites W2100509083 @default.
• W2081367861 cites W2103201992 @default.
• W2081367861 cites W2104342221 @default.
• W2081367861 cites W2111835763 @default.
• W2081367861 cites W2115294768 @default.
• W2081367861 cites W2117200840 @default.
• W2081367861 cites W2121744671 @default.
• W2081367861 cites W2124154627 @default.
• W2081367861 cites W2136280424 @default.
• W2081367861 cites W2143464311 @default.
• W2081367861 cites W2154568166 @default.
• W2081367861 cites W2154853638 @default.
• W2081367861 cites W2164615751 @default.
• W2081367861 cites W2164733896 @default.
• W2081367861 cites W4235438234 @default.
• W2081367861 cites W4238507985 @default.
• W2081367861 cites W95789664 @default.
• W2081367861 doi "https://doi.org/10.1074/jbc.m114.568501" @default.
• W2081367861 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4140886" @default.
• W2081367861 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24727589" @default.
• W2081367861 hasPublicationYear "2014" @default.
• W2081367861 type Work @default.
• W2081367861 sameAs 2081367861 @default.
• W2081367861 citedByCount "25" @default.
• W2081367861 countsByYear W20813678612014 @default.
• W2081367861 countsByYear W20813678612015 @default.
• W2081367861 countsByYear W20813678612016 @default.
• W2081367861 countsByYear W20813678612017 @default.
• W2081367861 countsByYear W20813678612018 @default.
• W2081367861 countsByYear W20813678612019 @default.
• W2081367861 countsByYear W20813678612020 @default.
• W2081367861 countsByYear W20813678612021 @default.
• W2081367861 countsByYear W20813678612023 @default.
• W2081367861 crossrefType "journal-article" @default.
• W2081367861 hasAuthorship W2081367861A5010483841 @default.
• W2081367861 hasAuthorship W2081367861A5087042183 @default.
• W2081367861 hasBestOaLocation W20813678611 @default.
• W2081367861 hasConcept C111798993 @default.
• W2081367861 hasConcept C118131993 @default.
• W2081367861 hasConcept C121608353 @default.
• W2081367861 hasConcept C153911025 @default.
• W2081367861 hasConcept C2777658100 @default.
• W2081367861 hasConcept C2780381497 @default.
• W2081367861 hasConcept C502942594 @default.
• W2081367861 hasConcept C54355233 @default.
• W2081367861 hasConcept C81885089 @default.
• W2081367861 hasConcept C86803240 @default.
• W2081367861 hasConcept C95444343 @default.
• W2081367861 hasConceptScore W2081367861C111798993 @default.
• W2081367861 hasConceptScore W2081367861C118131993 @default.
• W2081367861 hasConceptScore W2081367861C121608353 @default.
• W2081367861 hasConceptScore W2081367861C153911025 @default.

• next