Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081382012> ?p ?o ?g. }
- W2081382012 endingPage "1622" @default.
- W2081382012 startingPage "1610" @default.
- W2081382012 abstract "Accurate and efficient processing of pre-rRNA is critical to the accumulation of mature functional ribosomal subunits for maintenance of cell growth. Processing requires numerous factors which act in trans as well as RNA sequence/structural elements which function in cis. To examine the latter, we have used directed mutagenesis and expression of mutated pre-rRNAs in yeast. Specifically, we tested requirements for formation of an ITS2-proximal stem on processing, a structure formed by an interaction between sequences corresponding to the 3′ end of 5.8S rRNA and the 5′ end of 25S. Pre-rRNA processing is inhibited in templates encoding mutations that prevent the formation of the ITS2-proximal stem. Compensatory, double mutations, which alter the sequence of this region but restore the structure of the stem, also restore processing, although at lower efficiency. This reduction in efficiency is reflected in decreased levels of mature 5.8S and 25S rRNA and increased levels of 35S pre-rRNA and certain processing intermediates. This phenotype is reminiscent of the biochemical depletion of U8 snoRNA in vertebrates for which the ITS2-proximal stem has been proposed as a potential site for interaction with U8 RNP. Thus, formation of the ITS2-proximal stem may be a requirement common to yeast and vertebrate pre-rRNA processing." @default.
- W2081382012 created "2016-06-24" @default.
- W2081382012 creator A5054081266 @default.
- W2081382012 creator A5069347548 @default.
- W2081382012 date "1998-12-01" @default.
- W2081382012 modified "2023-09-24" @default.
- W2081382012 title "The structure of the ITS2-proximal stem is required for pre-rRNA processing in yeast" @default.
- W2081382012 cites W109701900 @default.
- W2081382012 cites W1497058365 @default.
- W2081382012 cites W1546922418 @default.
- W2081382012 cites W1585970141 @default.
- W2081382012 cites W169537812 @default.
- W2081382012 cites W1846812271 @default.
- W2081382012 cites W1851539110 @default.
- W2081382012 cites W1903554530 @default.
- W2081382012 cites W1929480046 @default.
- W2081382012 cites W1965401154 @default.
- W2081382012 cites W1966929898 @default.
- W2081382012 cites W1981260361 @default.
- W2081382012 cites W1984491679 @default.
- W2081382012 cites W1988479344 @default.
- W2081382012 cites W1996609063 @default.
- W2081382012 cites W1996851109 @default.
- W2081382012 cites W2002829809 @default.
- W2081382012 cites W2005447120 @default.
- W2081382012 cites W2026207708 @default.
- W2081382012 cites W2032678548 @default.
- W2081382012 cites W2034307038 @default.
- W2081382012 cites W2045913549 @default.
- W2081382012 cites W2054175147 @default.
- W2081382012 cites W2055348787 @default.
- W2081382012 cites W2060299254 @default.
- W2081382012 cites W2071436812 @default.
- W2081382012 cites W2074573090 @default.
- W2081382012 cites W2077276679 @default.
- W2081382012 cites W2080055531 @default.
- W2081382012 cites W2087436914 @default.
- W2081382012 cites W2097343134 @default.
- W2081382012 cites W2099158977 @default.
- W2081382012 cites W2099507653 @default.
- W2081382012 cites W2109422606 @default.
- W2081382012 cites W2109872488 @default.
- W2081382012 cites W2126139416 @default.
- W2081382012 cites W2138329420 @default.
- W2081382012 cites W2140224280 @default.
- W2081382012 cites W2144535541 @default.
- W2081382012 cites W2153283265 @default.
- W2081382012 cites W2169618180 @default.
- W2081382012 cites W2290177283 @default.
- W2081382012 cites W2292762434 @default.
- W2081382012 cites W2396066281 @default.
- W2081382012 cites W260776168 @default.
- W2081382012 cites W2993518094 @default.
- W2081382012 doi "https://doi.org/10.1017/s1355838298981420" @default.
- W2081382012 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1369729" @default.
- W2081382012 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9848657" @default.
- W2081382012 hasPublicationYear "1998" @default.
- W2081382012 type Work @default.
- W2081382012 sameAs 2081382012 @default.
- W2081382012 citedByCount "47" @default.
- W2081382012 countsByYear W20813820122012 @default.
- W2081382012 countsByYear W20813820122013 @default.
- W2081382012 countsByYear W20813820122014 @default.
- W2081382012 countsByYear W20813820122015 @default.
- W2081382012 countsByYear W20813820122016 @default.
- W2081382012 countsByYear W20813820122017 @default.
- W2081382012 countsByYear W20813820122018 @default.
- W2081382012 countsByYear W20813820122020 @default.
- W2081382012 countsByYear W20813820122021 @default.
- W2081382012 countsByYear W20813820122022 @default.
- W2081382012 crossrefType "journal-article" @default.
- W2081382012 hasAuthorship W2081382012A5054081266 @default.
- W2081382012 hasAuthorship W2081382012A5069347548 @default.
- W2081382012 hasBestOaLocation W20813820122 @default.
- W2081382012 hasConcept C104317684 @default.
- W2081382012 hasConcept C16318435 @default.
- W2081382012 hasConcept C179255354 @default.
- W2081382012 hasConcept C194993378 @default.
- W2081382012 hasConcept C2779222958 @default.
- W2081382012 hasConcept C501734568 @default.
- W2081382012 hasConcept C54355233 @default.
- W2081382012 hasConcept C67705224 @default.
- W2081382012 hasConcept C67905577 @default.
- W2081382012 hasConcept C70721500 @default.
- W2081382012 hasConcept C86803240 @default.
- W2081382012 hasConcept C95444343 @default.
- W2081382012 hasConceptScore W2081382012C104317684 @default.
- W2081382012 hasConceptScore W2081382012C16318435 @default.
- W2081382012 hasConceptScore W2081382012C179255354 @default.
- W2081382012 hasConceptScore W2081382012C194993378 @default.
- W2081382012 hasConceptScore W2081382012C2779222958 @default.
- W2081382012 hasConceptScore W2081382012C501734568 @default.
- W2081382012 hasConceptScore W2081382012C54355233 @default.
- W2081382012 hasConceptScore W2081382012C67705224 @default.
- W2081382012 hasConceptScore W2081382012C67905577 @default.
- W2081382012 hasConceptScore W2081382012C70721500 @default.
- W2081382012 hasConceptScore W2081382012C86803240 @default.
- W2081382012 hasConceptScore W2081382012C95444343 @default.