FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081451897> ?p ?o ?g. }

• W2081451897 endingPage "1675" @default.
• W2081451897 startingPage "1667" @default.
• W2081451897 abstract "Hoechst 33342 (H342) in combination with ultraviolet (UV) irradiation is frequently used to assist the enucleation of porcine oocytes in somatic cell nuclear transfer programs. This work evaluated the effects of H342 (5 μg/mL for 12 min) staining and/or exposure to UV irradiation on fertilisability and developmental capacity of porcine oocytes matured in vitro. In Experiment 1, a total of 1388 mature oocytes were distributed in the following groups: Group 1: oocytes without treatment (Control), Group 2: oocytes stained with H342, Group 3: oocytes stained with H342 and UV irradiated for 30 sec, and Group 4: oocytes UV irradiated for 30 sec. Oocytes from each group were exposed to thawed spermatozoa and cultured for 18 h to assess fertilization parameters or for 7 d to evaluate embryo development. Sperm penetration (P < 0.001) and monospermy (P < 0.04) were lower in oocytes exposed to H342/UV (80.7 ± 4.5% and 30.7 ± 5.4%, respectively) than in oocytes from the control group (94.9 ± 4.3 and 50.0 ± 4.9, respectively). The oocytes exposed to H342/UV showed lower (P < 0.001) cleavage (49.8 ± 2.9%) and blastocyst (7.7 ± 2.9%) rates than oocytes from the other groups (range: 73.8 ± 2.9% to 77.7 ± 2.9% and 22.3 ± 2.9% to 30.9 ± 3.0%, respectively). Experiment 2 was designed to evaluate the effect of shorter UV irradiation (5 sec). A total of 1835 mature oocytes were separated into the same groups as those of Experiment 1. The fertilization parameters and the cleavage rates were not influenced by the different treatments. However, the oocytes exposed to H342 and UV irradiation for 5 sec showed a lower (P < 0.02) rate of blastocyst formation (15.2 ± 4.5%) than the oocytes from other groups (range: 26.1 ± 4.5% to 30.7 ± 4.5%). In conclusion, our results demonstrate that the combination of H342 staining with UV irradiation has a clear deleterious effect on the developmental ability of oocytes, with the effects being more intense with increased exposure to UV irradiation." @default.
• W2081451897 created "2016-06-24" @default.
• W2081451897 creator A5008849004 @default.
• W2081451897 creator A5011323287 @default.
• W2081451897 creator A5034599887 @default.
• W2081451897 creator A5035272186 @default.
• W2081451897 creator A5041220963 @default.
• W2081451897 creator A5067017120 @default.
• W2081451897 creator A5069158582 @default.
• W2081451897 creator A5070457912 @default.
• W2081451897 creator A5084996489 @default.
• W2081451897 creator A5086899576 @default.
• W2081451897 date "2011-12-01" @default.
• W2081451897 modified "2023-10-17" @default.
• W2081451897 title "Effects of Hoechst 33342 staining and ultraviolet irradiation on the developmental competence of in vitro-matured porcine oocytes" @default.
• W2081451897 cites W1549680994 @default.
• W2081451897 cites W1688752194 @default.
• W2081451897 cites W1916885428 @default.
• W2081451897 cites W1969345130 @default.
• W2081451897 cites W1987247569 @default.
• W2081451897 cites W2000857607 @default.
• W2081451897 cites W2003607285 @default.
• W2081451897 cites W2005761200 @default.
• W2081451897 cites W2020393095 @default.
• W2081451897 cites W2021477641 @default.
• W2081451897 cites W2024887000 @default.
• W2081451897 cites W2025960524 @default.
• W2081451897 cites W2038358710 @default.
• W2081451897 cites W2042029389 @default.
• W2081451897 cites W2055361521 @default.
• W2081451897 cites W2059772554 @default.
• W2081451897 cites W2063327534 @default.
• W2081451897 cites W2093311258 @default.
• W2081451897 cites W2093830134 @default.
• W2081451897 cites W2106509794 @default.
• W2081451897 cites W2122907791 @default.
• W2081451897 cites W2127775724 @default.
• W2081451897 cites W2128246174 @default.
• W2081451897 cites W2133433022 @default.
• W2081451897 cites W2144811676 @default.
• W2081451897 cites W2145678727 @default.
• W2081451897 cites W2154668036 @default.
• W2081451897 cites W2160485770 @default.
• W2081451897 cites W2168524056 @default.
• W2081451897 cites W2168587876 @default.
• W2081451897 doi "https://doi.org/10.1016/j.theriogenology.2011.06.032" @default.
• W2081451897 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21872317" @default.
• W2081451897 hasPublicationYear "2011" @default.
• W2081451897 type Work @default.
• W2081451897 sameAs 2081451897 @default.
• W2081451897 citedByCount "11" @default.
• W2081451897 countsByYear W20814518972012 @default.
• W2081451897 countsByYear W20814518972013 @default.
• W2081451897 countsByYear W20814518972014 @default.
• W2081451897 countsByYear W20814518972016 @default.
• W2081451897 countsByYear W20814518972018 @default.
• W2081451897 countsByYear W20814518972020 @default.
• W2081451897 countsByYear W20814518972023 @default.
• W2081451897 crossrefType "journal-article" @default.
• W2081451897 hasAuthorship W2081451897A5008849004 @default.
• W2081451897 hasAuthorship W2081451897A5011323287 @default.
• W2081451897 hasAuthorship W2081451897A5034599887 @default.
• W2081451897 hasAuthorship W2081451897A5035272186 @default.
• W2081451897 hasAuthorship W2081451897A5041220963 @default.
• W2081451897 hasAuthorship W2081451897A5067017120 @default.
• W2081451897 hasAuthorship W2081451897A5069158582 @default.
• W2081451897 hasAuthorship W2081451897A5070457912 @default.
• W2081451897 hasAuthorship W2081451897A5084996489 @default.
• W2081451897 hasAuthorship W2081451897A5086899576 @default.
• W2081451897 hasConcept C105702510 @default.
• W2081451897 hasConcept C111337013 @default.
• W2081451897 hasConcept C121332964 @default.
• W2081451897 hasConcept C16685009 @default.
• W2081451897 hasConcept C185544564 @default.
• W2081451897 hasConcept C185592680 @default.
• W2081451897 hasConcept C188261085 @default.
• W2081451897 hasConcept C196843134 @default.
• W2081451897 hasConcept C2776690073 @default.
• W2081451897 hasConcept C2778177303 @default.
• W2081451897 hasConcept C2781087480 @default.
• W2081451897 hasConcept C512716672 @default.
• W2081451897 hasConcept C54355233 @default.
• W2081451897 hasConcept C71924100 @default.
• W2081451897 hasConcept C74864618 @default.
• W2081451897 hasConcept C86803240 @default.
• W2081451897 hasConcept C87073359 @default.
• W2081451897 hasConcept C88972607 @default.
• W2081451897 hasConceptScore W2081451897C105702510 @default.
• W2081451897 hasConceptScore W2081451897C111337013 @default.
• W2081451897 hasConceptScore W2081451897C121332964 @default.
• W2081451897 hasConceptScore W2081451897C16685009 @default.
• W2081451897 hasConceptScore W2081451897C185544564 @default.
• W2081451897 hasConceptScore W2081451897C185592680 @default.
• W2081451897 hasConceptScore W2081451897C188261085 @default.
• W2081451897 hasConceptScore W2081451897C196843134 @default.
• W2081451897 hasConceptScore W2081451897C2776690073 @default.
• W2081451897 hasConceptScore W2081451897C2778177303 @default.
• W2081451897 hasConceptScore W2081451897C2781087480 @default.

• next