Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081464109> ?p ?o ?g. }
- W2081464109 abstract "Abstract Two isoforms of glutamine synthetase were identified from Canavalia lineata leaves and two forms (designated GS1 and GS2) showed different properties. The molecular weight estimated by gel filtration was 340 kDa for the GS1 and 407 kDa for the GS2. The optimum pH of the GS2 (pH 8.0) was somewhat higher than that of the GS1 (pH 7.5), while, the activation energies and the optimum temperatures of the two isoforms were similar. The inhibition patterns of the isoforms by methionine sulfoximine were different from each other: The competitive inhibition for the GS1 with a very low Kis value of 0.033 mM was compared to the non-competitive inhibition for the GS2 with a Kis value of 0.27 mM and a Kii value of 0.17 mM. The result of the initial velocity studies for the isoenzymes was compatible with a sequential-random kinetic mechanism. The kinetic parameters at 35°C and pH 7.8 were KGlu=5.29 mM, KATP=0.24 mM, and KHydroxylamine=0.07 mM for the GS1 and KGlu=2.14 mM, KATP=0.57 mM, and KHydroxylamine=0.09 mM for the GS2. The Viola and Cleland's method used in this kinetic analysis was more exact and more acceptable than other methods used until now to analyze the glutamine synthetase. The molecular weight of GS2 purified with Cibacron blue dye chromatography was 42 kDa and the anti-GS2 antibody could detect three different forms of GS subunit in different organs." @default.
- W2081464109 created "2016-06-24" @default.
- W2081464109 creator A5075579017 @default.
- W2081464109 creator A5077891562 @default.
- W2081464109 date "1998-06-01" @default.
- W2081464109 modified "2023-09-23" @default.
- W2081464109 title "Comparative characterization of glutamine synthetase isoforms from Canavalia lineata in biochemical and immunological aspects" @default.
- W2081464109 cites W1491512510 @default.
- W2081464109 cites W1540272106 @default.
- W2081464109 cites W1553288841 @default.
- W2081464109 cites W1562390152 @default.
- W2081464109 cites W1573147813 @default.
- W2081464109 cites W1601712037 @default.
- W2081464109 cites W1687446504 @default.
- W2081464109 cites W1966343435 @default.
- W2081464109 cites W1977660801 @default.
- W2081464109 cites W1990829921 @default.
- W2081464109 cites W1993404602 @default.
- W2081464109 cites W1999475750 @default.
- W2081464109 cites W2012233583 @default.
- W2081464109 cites W2025914057 @default.
- W2081464109 cites W2032550826 @default.
- W2081464109 cites W2043351145 @default.
- W2081464109 cites W2043865991 @default.
- W2081464109 cites W2067096639 @default.
- W2081464109 cites W2068524830 @default.
- W2081464109 cites W2071647197 @default.
- W2081464109 cites W2073637469 @default.
- W2081464109 cites W2076087943 @default.
- W2081464109 cites W2077996833 @default.
- W2081464109 cites W2085689680 @default.
- W2081464109 cites W2115223771 @default.
- W2081464109 cites W2128718773 @default.
- W2081464109 cites W2150377117 @default.
- W2081464109 cites W2165033666 @default.
- W2081464109 cites W2202951980 @default.
- W2081464109 cites W2220757509 @default.
- W2081464109 cites W2273557250 @default.
- W2081464109 cites W255756636 @default.
- W2081464109 cites W2990537868 @default.
- W2081464109 cites W3017247983 @default.
- W2081464109 doi "https://doi.org/10.1016/s0168-9452(98)00059-4" @default.
- W2081464109 hasPublicationYear "1998" @default.
- W2081464109 type Work @default.
- W2081464109 sameAs 2081464109 @default.
- W2081464109 citedByCount "4" @default.
- W2081464109 countsByYear W20814641092012 @default.
- W2081464109 crossrefType "journal-article" @default.
- W2081464109 hasAuthorship W2081464109A5075579017 @default.
- W2081464109 hasAuthorship W2081464109A5077891562 @default.
- W2081464109 hasConcept C104292427 @default.
- W2081464109 hasConcept C104317684 @default.
- W2081464109 hasConcept C119795356 @default.
- W2081464109 hasConcept C150757390 @default.
- W2081464109 hasConcept C153911025 @default.
- W2081464109 hasConcept C181199279 @default.
- W2081464109 hasConcept C185592680 @default.
- W2081464109 hasConcept C193363816 @default.
- W2081464109 hasConcept C2779289688 @default.
- W2081464109 hasConcept C2779349466 @default.
- W2081464109 hasConcept C43617362 @default.
- W2081464109 hasConcept C515207424 @default.
- W2081464109 hasConcept C53345823 @default.
- W2081464109 hasConcept C55493867 @default.
- W2081464109 hasConcept C58121356 @default.
- W2081464109 hasConcept C86803240 @default.
- W2081464109 hasConceptScore W2081464109C104292427 @default.
- W2081464109 hasConceptScore W2081464109C104317684 @default.
- W2081464109 hasConceptScore W2081464109C119795356 @default.
- W2081464109 hasConceptScore W2081464109C150757390 @default.
- W2081464109 hasConceptScore W2081464109C153911025 @default.
- W2081464109 hasConceptScore W2081464109C181199279 @default.
- W2081464109 hasConceptScore W2081464109C185592680 @default.
- W2081464109 hasConceptScore W2081464109C193363816 @default.
- W2081464109 hasConceptScore W2081464109C2779289688 @default.
- W2081464109 hasConceptScore W2081464109C2779349466 @default.
- W2081464109 hasConceptScore W2081464109C43617362 @default.
- W2081464109 hasConceptScore W2081464109C515207424 @default.
- W2081464109 hasConceptScore W2081464109C53345823 @default.
- W2081464109 hasConceptScore W2081464109C55493867 @default.
- W2081464109 hasConceptScore W2081464109C58121356 @default.
- W2081464109 hasConceptScore W2081464109C86803240 @default.
- W2081464109 hasLocation W20814641091 @default.
- W2081464109 hasOpenAccess W2081464109 @default.
- W2081464109 hasPrimaryLocation W20814641091 @default.
- W2081464109 hasRelatedWork W1967957191 @default.
- W2081464109 hasRelatedWork W1986867704 @default.
- W2081464109 hasRelatedWork W1999475750 @default.
- W2081464109 hasRelatedWork W1999877847 @default.
- W2081464109 hasRelatedWork W2006336924 @default.
- W2081464109 hasRelatedWork W2015560870 @default.
- W2081464109 hasRelatedWork W2016527764 @default.
- W2081464109 hasRelatedWork W2017990519 @default.
- W2081464109 hasRelatedWork W2024803616 @default.
- W2081464109 hasRelatedWork W2058388916 @default.
- W2081464109 hasRelatedWork W2064564403 @default.
- W2081464109 hasRelatedWork W2068796954 @default.
- W2081464109 hasRelatedWork W2082750929 @default.
- W2081464109 hasRelatedWork W2089461021 @default.
- W2081464109 hasRelatedWork W2111453675 @default.