Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081480985> ?p ?o ?g. }
- W2081480985 endingPage "535" @default.
- W2081480985 startingPage "529" @default.
- W2081480985 abstract "The effect of hydrostatic pressure on the secondary structure of recombinant human interferon-γ (rhIFN-γ) and its biologically inactive truncated form rhIFN-ΔC15 has been studied using Fourier-transform IR (FTIR) spectroscopy. In situ observation of the pressure-induced changes using the diamond anvil cell shows that the α-helical structure is mainly transformed into disordered structure at high pressure. Increasing pressure also induces the formation of a gel. Addition of 0.5M MgCl2 significantly reduces the pressure stability. Releasing the pressure below 300MPa results in the formation of intermolecular antiparallel β-sheets, which is seldom observed. This suggests that the intermolecular β-sheet of rhIFN-γ is stabilized by electrostatic interactions that are disrupted at high pressure. For comparison we also studied the effect of temperature. Temperature-induced changes reflect extensive transformation of α-helical structure into intermolecular antiparallel β-sheet, as is usually observed for most proteins." @default.
- W2081480985 created "2016-06-24" @default.
- W2081480985 creator A5009998598 @default.
- W2081480985 creator A5025659584 @default.
- W2081480985 creator A5043456455 @default.
- W2081480985 creator A5059957944 @default.
- W2081480985 creator A5074552437 @default.
- W2081480985 date "2003-03-01" @default.
- W2081480985 modified "2023-10-18" @default.
- W2081480985 title "Pressure- and temperature-induced unfolding and aggregation of recombinant human interferon-gamma: a Fourier transform infrared spectroscopy study" @default.
- W2081480985 cites W1533862222 @default.
- W2081480985 cites W1967073845 @default.
- W2081480985 cites W1969880312 @default.
- W2081480985 cites W1980469335 @default.
- W2081480985 cites W1982288755 @default.
- W2081480985 cites W1987592999 @default.
- W2081480985 cites W2001461112 @default.
- W2081480985 cites W2005694227 @default.
- W2081480985 cites W2008131887 @default.
- W2081480985 cites W2010735466 @default.
- W2081480985 cites W2015512183 @default.
- W2081480985 cites W2016989643 @default.
- W2081480985 cites W2023091090 @default.
- W2081480985 cites W2030808787 @default.
- W2081480985 cites W2037193428 @default.
- W2081480985 cites W2041871841 @default.
- W2081480985 cites W2044208863 @default.
- W2081480985 cites W2045389690 @default.
- W2081480985 cites W2056701468 @default.
- W2081480985 cites W2061409649 @default.
- W2081480985 cites W2061931208 @default.
- W2081480985 cites W2062102053 @default.
- W2081480985 cites W2064431338 @default.
- W2081480985 cites W2066976694 @default.
- W2081480985 cites W2071191299 @default.
- W2081480985 cites W2076953122 @default.
- W2081480985 cites W2077231187 @default.
- W2081480985 cites W2088025115 @default.
- W2081480985 cites W2095450147 @default.
- W2081480985 cites W2096946406 @default.
- W2081480985 cites W2097703618 @default.
- W2081480985 cites W2105360761 @default.
- W2081480985 cites W2111726538 @default.
- W2081480985 cites W2125016536 @default.
- W2081480985 cites W2135779527 @default.
- W2081480985 cites W2141250779 @default.
- W2081480985 cites W2141654481 @default.
- W2081480985 cites W2144985982 @default.
- W2081480985 cites W2153439292 @default.
- W2081480985 cites W2155916246 @default.
- W2081480985 cites W2229489099 @default.
- W2081480985 cites W2417757184 @default.
- W2081480985 cites W296432340 @default.
- W2081480985 doi "https://doi.org/10.1042/bj20020717" @default.
- W2081480985 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1223177" @default.
- W2081480985 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12425720" @default.
- W2081480985 hasPublicationYear "2003" @default.
- W2081480985 type Work @default.
- W2081480985 sameAs 2081480985 @default.
- W2081480985 citedByCount "30" @default.
- W2081480985 countsByYear W20814809852012 @default.
- W2081480985 countsByYear W20814809852013 @default.
- W2081480985 countsByYear W20814809852014 @default.
- W2081480985 countsByYear W20814809852015 @default.
- W2081480985 countsByYear W20814809852017 @default.
- W2081480985 countsByYear W20814809852018 @default.
- W2081480985 countsByYear W20814809852019 @default.
- W2081480985 countsByYear W20814809852022 @default.
- W2081480985 countsByYear W20814809852023 @default.
- W2081480985 crossrefType "journal-article" @default.
- W2081480985 hasAuthorship W2081480985A5009998598 @default.
- W2081480985 hasAuthorship W2081480985A5025659584 @default.
- W2081480985 hasAuthorship W2081480985A5043456455 @default.
- W2081480985 hasAuthorship W2081480985A5059957944 @default.
- W2081480985 hasAuthorship W2081480985A5074552437 @default.
- W2081480985 hasBestOaLocation W20814809852 @default.
- W2081480985 hasConcept C104317684 @default.
- W2081480985 hasConcept C113196181 @default.
- W2081480985 hasConcept C115260700 @default.
- W2081480985 hasConcept C121332964 @default.
- W2081480985 hasConcept C12554922 @default.
- W2081480985 hasConcept C127413603 @default.
- W2081480985 hasConcept C142089489 @default.
- W2081480985 hasConcept C153642686 @default.
- W2081480985 hasConcept C160892712 @default.
- W2081480985 hasConcept C166950319 @default.
- W2081480985 hasConcept C178790620 @default.
- W2081480985 hasConcept C185592680 @default.
- W2081480985 hasConcept C2910081258 @default.
- W2081480985 hasConcept C32891209 @default.
- W2081480985 hasConcept C32909587 @default.
- W2081480985 hasConcept C40767141 @default.
- W2081480985 hasConcept C42360764 @default.
- W2081480985 hasConcept C55493867 @default.
- W2081480985 hasConcept C62520636 @default.
- W2081480985 hasConcept C8010536 @default.
- W2081480985 hasConcept C86803240 @default.
- W2081480985 hasConcept C97355855 @default.