Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081503886> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2081503886 endingPage "3343" @default.
- W2081503886 startingPage "3339" @default.
- W2081503886 abstract "Single base pair differences between otherwise identical DNA molecules can result in altered melting behavior detectable by denaturing gradient gel electrophoresis. We have developed a simplified procedure for using denaturing gradient gel electrophoresis to detect base pair changes in genomic DNA. Genomic DNA is digested with restriction enzymes and hybridized in solution to labeled single-stranded probe DNA. The excess probe is then hybridized to complementary phage M13 template DNA, and the reaction mixture is electrophoresed on a denaturing gradient gel. Only the genomic DNA probe hybrids migrate into the gel. Differences in hybrid mobility on the gel indicate base pair changes in the genomic DNA. We have used this technique to identify two polymorphic sites within a 1.2-kilobase region of human chromosome 20. This approach should greatly facilitate the identification of DNA polymorphisms useful for gene linkage studies and the diagnosis of genetic diseases." @default.
- W2081503886 created "2016-06-24" @default.
- W2081503886 creator A5077361637 @default.
- W2081503886 creator A5084750972 @default.
- W2081503886 date "1987-05-01" @default.
- W2081503886 modified "2023-10-16" @default.
- W2081503886 title "Detection of human DNA polymorphisms with a simplified denaturing gradient gel electrophoresis technique." @default.
- W2081503886 cites W1538298016 @default.
- W2081503886 cites W1967104104 @default.
- W2081503886 cites W1992368452 @default.
- W2081503886 cites W2031213896 @default.
- W2081503886 cites W2092765135 @default.
- W2081503886 cites W2136861317 @default.
- W2081503886 cites W2172960123 @default.
- W2081503886 cites W2431261839 @default.
- W2081503886 cites W938733668 @default.
- W2081503886 doi "https://doi.org/10.1073/pnas.84.10.3339" @default.
- W2081503886 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/304865" @default.
- W2081503886 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2883652" @default.
- W2081503886 hasPublicationYear "1987" @default.
- W2081503886 type Work @default.
- W2081503886 sameAs 2081503886 @default.
- W2081503886 citedByCount "36" @default.
- W2081503886 countsByYear W20815038862012 @default.
- W2081503886 countsByYear W20815038862019 @default.
- W2081503886 crossrefType "journal-article" @default.
- W2081503886 hasAuthorship W2081503886A5077361637 @default.
- W2081503886 hasAuthorship W2081503886A5084750972 @default.
- W2081503886 hasBestOaLocation W20815038861 @default.
- W2081503886 hasConcept C104317684 @default.
- W2081503886 hasConcept C116084860 @default.
- W2081503886 hasConcept C128319531 @default.
- W2081503886 hasConcept C153911025 @default.
- W2081503886 hasConcept C17757408 @default.
- W2081503886 hasConcept C185581394 @default.
- W2081503886 hasConcept C198549448 @default.
- W2081503886 hasConcept C3017666073 @default.
- W2081503886 hasConcept C42062724 @default.
- W2081503886 hasConcept C54355233 @default.
- W2081503886 hasConcept C54871578 @default.
- W2081503886 hasConcept C552990157 @default.
- W2081503886 hasConcept C84289048 @default.
- W2081503886 hasConcept C86803240 @default.
- W2081503886 hasConceptScore W2081503886C104317684 @default.
- W2081503886 hasConceptScore W2081503886C116084860 @default.
- W2081503886 hasConceptScore W2081503886C128319531 @default.
- W2081503886 hasConceptScore W2081503886C153911025 @default.
- W2081503886 hasConceptScore W2081503886C17757408 @default.
- W2081503886 hasConceptScore W2081503886C185581394 @default.
- W2081503886 hasConceptScore W2081503886C198549448 @default.
- W2081503886 hasConceptScore W2081503886C3017666073 @default.
- W2081503886 hasConceptScore W2081503886C42062724 @default.
- W2081503886 hasConceptScore W2081503886C54355233 @default.
- W2081503886 hasConceptScore W2081503886C54871578 @default.
- W2081503886 hasConceptScore W2081503886C552990157 @default.
- W2081503886 hasConceptScore W2081503886C84289048 @default.
- W2081503886 hasConceptScore W2081503886C86803240 @default.
- W2081503886 hasIssue "10" @default.
- W2081503886 hasLocation W20815038861 @default.
- W2081503886 hasLocation W20815038862 @default.
- W2081503886 hasLocation W20815038863 @default.
- W2081503886 hasLocation W20815038864 @default.
- W2081503886 hasOpenAccess W2081503886 @default.
- W2081503886 hasPrimaryLocation W20815038861 @default.
- W2081503886 hasRelatedWork W1999815997 @default.
- W2081503886 hasRelatedWork W2025365677 @default.
- W2081503886 hasRelatedWork W2048158576 @default.
- W2081503886 hasRelatedWork W2050077019 @default.
- W2081503886 hasRelatedWork W2080266123 @default.
- W2081503886 hasRelatedWork W2081503886 @default.
- W2081503886 hasRelatedWork W2145368198 @default.
- W2081503886 hasRelatedWork W2158738420 @default.
- W2081503886 hasRelatedWork W2341696882 @default.
- W2081503886 hasRelatedWork W2033365491 @default.
- W2081503886 hasVolume "84" @default.
- W2081503886 isParatext "false" @default.
- W2081503886 isRetracted "false" @default.
- W2081503886 magId "2081503886" @default.
- W2081503886 workType "article" @default.