FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081505855> ?p ?o ?g. }

• W2081505855 endingPage "2169.e8" @default.
• W2081505855 startingPage "2158" @default.
• W2081505855 abstract "Background & AimsHepatocyte-like cells can be derived from pluripotent stem cells such as embryonic stem (ES) cells, but ES cell-derived hepatic cells with extensive capacity to repopulate liver have not been identified. We aimed to identify and purify ES cell-derived hepatoblast-like progenitor cells and to explore their capacity for liver repopulation in mice after in vitro expansion.MethodsUnmanipulated mouse ES cells were cultured under defined conditions and allowed to undergo stepwise hepatic differentiation. The derived hepatic cells were examined by morphologic, fluorescence-activated cell sorting, gene expression, and clonal expansion analyses. The capacities of ES cell-derived hepatic progenitor cells to repopulate liver were investigated in mice that were deficient in fumarylacetoacetate hydrolase (Fah) (a model of liver injury).ResultsMouse ES cells were induced to differentiate into a population that contained hepatic progenitor cells; this population included cells that expressed epithelial cell adhesion molecule (EpCAM) but did not express c-Kit. Clonal hepatic progenitors that arose from single c-Kit−EpCAM+ cells could undergo long-term expansion and maintain hepatoblast-like characteristics. Enriched c-Kit−EpCAM+ cells and clonally expanded hepatic progenitor cells repopulated the livers of Fah-deficient mice without inducing tumorigenesis.ConclusionsES cell-derived c-Kit−EpCAM+ cells contain a population of hepatoblast-like progenitor cells that can repopulate livers of mice. Hepatocyte-like cells can be derived from pluripotent stem cells such as embryonic stem (ES) cells, but ES cell-derived hepatic cells with extensive capacity to repopulate liver have not been identified. We aimed to identify and purify ES cell-derived hepatoblast-like progenitor cells and to explore their capacity for liver repopulation in mice after in vitro expansion. Unmanipulated mouse ES cells were cultured under defined conditions and allowed to undergo stepwise hepatic differentiation. The derived hepatic cells were examined by morphologic, fluorescence-activated cell sorting, gene expression, and clonal expansion analyses. The capacities of ES cell-derived hepatic progenitor cells to repopulate liver were investigated in mice that were deficient in fumarylacetoacetate hydrolase (Fah) (a model of liver injury). Mouse ES cells were induced to differentiate into a population that contained hepatic progenitor cells; this population included cells that expressed epithelial cell adhesion molecule (EpCAM) but did not express c-Kit. Clonal hepatic progenitors that arose from single c-Kit−EpCAM+ cells could undergo long-term expansion and maintain hepatoblast-like characteristics. Enriched c-Kit−EpCAM+ cells and clonally expanded hepatic progenitor cells repopulated the livers of Fah-deficient mice without inducing tumorigenesis. ES cell-derived c-Kit−EpCAM+ cells contain a population of hepatoblast-like progenitor cells that can repopulate livers of mice." @default.
• W2081505855 created "2016-06-24" @default.
• W2081505855 creator A5001370323 @default.
• W2081505855 creator A5005786917 @default.
• W2081505855 creator A5006822602 @default.
• W2081505855 creator A5016113685 @default.
• W2081505855 creator A5016570969 @default.
• W2081505855 creator A5020133148 @default.
• W2081505855 creator A5027227070 @default.
• W2081505855 creator A5044441524 @default.
• W2081505855 creator A5047598001 @default.
• W2081505855 creator A5049475098 @default.
• W2081505855 creator A5053906669 @default.
• W2081505855 creator A5055054595 @default.
• W2081505855 creator A5055538318 @default.
• W2081505855 creator A5079813186 @default.
• W2081505855 creator A5082022764 @default.
• W2081505855 creator A5089231537 @default.
• W2081505855 date "2010-12-01" @default.
• W2081505855 modified "2023-10-10" @default.
• W2081505855 title "Hepatoblast-Like Progenitor Cells Derived From Embryonic Stem Cells Can Repopulate Livers of Mice" @default.
• W2081505855 cites W1965419330 @default.
• W2081505855 cites W1966450565 @default.
• W2081505855 cites W1975655671 @default.
• W2081505855 cites W1982786429 @default.
• W2081505855 cites W1986338679 @default.
• W2081505855 cites W1988801780 @default.
• W2081505855 cites W1994736606 @default.
• W2081505855 cites W1998666537 @default.
• W2081505855 cites W2000318995 @default.
• W2081505855 cites W2020755558 @default.
• W2081505855 cites W2027264065 @default.
• W2081505855 cites W2029573004 @default.
• W2081505855 cites W2047454701 @default.
• W2081505855 cites W2053355345 @default.
• W2081505855 cites W2060914434 @default.
• W2081505855 cites W2061288990 @default.
• W2081505855 cites W2062118486 @default.
• W2081505855 cites W2062984040 @default.
• W2081505855 cites W2068185076 @default.
• W2081505855 cites W2070700342 @default.
• W2081505855 cites W2089676626 @default.
• W2081505855 cites W2094689158 @default.
• W2081505855 cites W2103385665 @default.
• W2081505855 cites W2110335719 @default.
• W2081505855 cites W2120060713 @default.
• W2081505855 cites W2121054813 @default.
• W2081505855 cites W2122114596 @default.
• W2081505855 cites W2123128269 @default.
• W2081505855 cites W2123417462 @default.
• W2081505855 cites W2132556256 @default.
• W2081505855 cites W2136607014 @default.
• W2081505855 cites W2138977668 @default.
• W2081505855 cites W2143496279 @default.
• W2081505855 cites W2148247271 @default.
• W2081505855 cites W2155942576 @default.
• W2081505855 cites W2168967248 @default.
• W2081505855 doi "https://doi.org/10.1053/j.gastro.2010.08.042" @default.
• W2081505855 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20801124" @default.
• W2081505855 hasPublicationYear "2010" @default.
• W2081505855 type Work @default.
• W2081505855 sameAs 2081505855 @default.
• W2081505855 citedByCount "56" @default.
• W2081505855 countsByYear W20815058552012 @default.
• W2081505855 countsByYear W20815058552013 @default.
• W2081505855 countsByYear W20815058552014 @default.
• W2081505855 countsByYear W20815058552015 @default.
• W2081505855 countsByYear W20815058552016 @default.
• W2081505855 countsByYear W20815058552017 @default.
• W2081505855 countsByYear W20815058552018 @default.
• W2081505855 countsByYear W20815058552019 @default.
• W2081505855 countsByYear W20815058552020 @default.
• W2081505855 countsByYear W20815058552022 @default.
• W2081505855 countsByYear W20815058552023 @default.
• W2081505855 crossrefType "journal-article" @default.
• W2081505855 hasAuthorship W2081505855A5001370323 @default.
• W2081505855 hasAuthorship W2081505855A5005786917 @default.
• W2081505855 hasAuthorship W2081505855A5006822602 @default.
• W2081505855 hasAuthorship W2081505855A5016113685 @default.
• W2081505855 hasAuthorship W2081505855A5016570969 @default.
• W2081505855 hasAuthorship W2081505855A5020133148 @default.
• W2081505855 hasAuthorship W2081505855A5027227070 @default.
• W2081505855 hasAuthorship W2081505855A5044441524 @default.
• W2081505855 hasAuthorship W2081505855A5047598001 @default.
• W2081505855 hasAuthorship W2081505855A5049475098 @default.
• W2081505855 hasAuthorship W2081505855A5053906669 @default.
• W2081505855 hasAuthorship W2081505855A5055054595 @default.
• W2081505855 hasAuthorship W2081505855A5055538318 @default.
• W2081505855 hasAuthorship W2081505855A5079813186 @default.
• W2081505855 hasAuthorship W2081505855A5082022764 @default.
• W2081505855 hasAuthorship W2081505855A5089231537 @default.
• W2081505855 hasBestOaLocation W20815058551 @default.
• W2081505855 hasConcept C104317684 @default.
• W2081505855 hasConcept C107459253 @default.
• W2081505855 hasConcept C123012128 @default.
• W2081505855 hasConcept C144024400 @default.
• W2081505855 hasConcept C145103041 @default.
• W2081505855 hasConcept C149923435 @default.

• next