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- W2081562204 endingPage "2009" @default.
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- W2081562204 abstract "The Arabidopsis genome contains 17 predicted β-galactosidase genes, all of which belong to glycosyl hydrolase (GH) Family 35. These genes have been further grouped into seven subfamilies based on sequence similarity. The largest of these, subfamily a1, consists of six genes, Gal-1 (At3g13750), Gal-2 (At3g52840), Gal-3 (At4g36360), Gal-4 (At5g56870), Gal-5 (At1g45130), and Gal-12 (At4g26140), some of which were characterized in previous studies. We report here the purification and biochemical characterization of recombinant Gal-1, Gal-3, Gal-4 and Gal-12 from Pichia pastoris, completing the analysis of all six recombinant proteins, as well as the isolation and characterization of the native Gal-2 protein from Arabidopsis leaves. Comparison of the relative expression levels of the subfamily a1 β-galactosidases at the mRNA and protein levels uncovered evidence of differential regulation, which may involve post-transcriptional and post-translational processes. In addition, this study provides further support for the proposed function of the subfamily a1 β-galactosidases in cell wall modification based on analysis of the organ-specific expression and subcellular localization of Gal-1 and Gal-12. Our study suggests that, despite some differences in individual biochemical characteristics and expression patterns, each member of the family has the potential to contribute to the dynamics of the Arabidopsis plant cell wall." @default.
- W2081562204 created "2016-06-24" @default.
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- W2081562204 date "2009-12-01" @default.
- W2081562204 modified "2023-09-23" @default.
- W2081562204 title "Comparative characterization of the Arabidopsis subfamily a1 β-galactosidases" @default.
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- W2081562204 doi "https://doi.org/10.1016/j.phytochem.2009.08.008" @default.
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