FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081617320> ?p ?o ?g. }

• W2081617320 endingPage "429" @default.
• W2081617320 startingPage "422" @default.
• W2081617320 abstract "Abstract Induced succinate uptake and transport (defined as transport of a compound followed by its metabolism and transport in the absence of subsequent metabolism) by Pseudomonas putida are active processes resulting in intracellular succinate concentrations 10-fold that of the initial extracellular concentration. Uptake was studied with the wild-type strain P. putida P2 and transport with a mutant deficient in succinate dehydrogenase activity. Addition of succinate, fumarate, or malate to the growth medium induces both processes above a basal level. Induction is dependent on protein synthesis and subject to catabolite repression. When extracts of induced and noninduced wild-type cells were assayed for succinate dehydrogenase, fumarase, and malate dehydrogenase only malate dehydrogenase increased in specific activity. Transport is inhibited by iodoacetamide, KCN, NaN3, and 2,4-dinitrophenol and shows pH and temperature optima of 6.2 and 30 °C. Kinetic parameters are: basal uptake (cells grown on glutamate) Km 11.6 μ m , v 0.32 nmoles per min per mg dry cell mass; induced uptake (cells grown on succinate plus NH4Cl) Km 12.5 μ m , v 5.78 nmoles per min per mg dry cell mass; induced transport (cells grown on nutrient broth plus succinate) Km 10 μ m , V 0.98 nmoles per min per mg dry cell mass. It was not possible to determine the kinetic parameters of basal transport. Malate and fumarate were the only compounds exhibiting competitive inhibition of uptake and transport suggesting common transport system for all three compounds. The Ki values for competitive inhibition and the Km for succinate indicate the order of affinity for both uptake and transport are succinate > malate > fumarate. Data from kinetic parameters of uptake and transport and studies on succinate metabolism provide evidence consistent with concurrent increases in transport and metabolism to account for induced succinate uptake by P. putida." @default.
• W2081617320 created "2016-06-24" @default.
• W2081617320 creator A5007885908 @default.
• W2081617320 creator A5060953227 @default.
• W2081617320 creator A5091796380 @default.
• W2081617320 date "1974-02-01" @default.
• W2081617320 modified "2023-09-26" @default.
• W2081617320 title "Transport of succinate by pseudomonas putida" @default.
• W2081617320 cites W13213655 @default.
• W2081617320 cites W1480823958 @default.
• W2081617320 cites W1492105562 @default.
• W2081617320 cites W1495959547 @default.
• W2081617320 cites W1506043065 @default.
• W2081617320 cites W1538697650 @default.
• W2081617320 cites W1558385973 @default.
• W2081617320 cites W1866236445 @default.
• W2081617320 cites W1936143151 @default.
• W2081617320 cites W1982741152 @default.
• W2081617320 cites W2025234730 @default.
• W2081617320 cites W2039972313 @default.
• W2081617320 cites W2054979333 @default.
• W2081617320 cites W2105370599 @default.
• W2081617320 cites W2288593283 @default.
• W2081617320 cites W275039816 @default.
• W2081617320 cites W72290756 @default.
• W2081617320 doi "https://doi.org/10.1016/0003-9861(74)90416-0" @default.
• W2081617320 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4831622" @default.
• W2081617320 hasPublicationYear "1974" @default.
• W2081617320 type Work @default.
• W2081617320 sameAs 2081617320 @default.
• W2081617320 citedByCount "22" @default.
• W2081617320 crossrefType "journal-article" @default.
• W2081617320 hasAuthorship W2081617320A5007885908 @default.
• W2081617320 hasAuthorship W2081617320A5060953227 @default.
• W2081617320 hasAuthorship W2081617320A5091796380 @default.
• W2081617320 hasConcept C181199279 @default.
• W2081617320 hasConcept C185592680 @default.
• W2081617320 hasConcept C2780543182 @default.
• W2081617320 hasConcept C2781463711 @default.
• W2081617320 hasConcept C523546767 @default.
• W2081617320 hasConcept C54355233 @default.
• W2081617320 hasConcept C55493867 @default.
• W2081617320 hasConcept C86803240 @default.
• W2081617320 hasConcept C89423630 @default.
• W2081617320 hasConceptScore W2081617320C181199279 @default.
• W2081617320 hasConceptScore W2081617320C185592680 @default.
• W2081617320 hasConceptScore W2081617320C2780543182 @default.
• W2081617320 hasConceptScore W2081617320C2781463711 @default.
• W2081617320 hasConceptScore W2081617320C523546767 @default.
• W2081617320 hasConceptScore W2081617320C54355233 @default.
• W2081617320 hasConceptScore W2081617320C55493867 @default.
• W2081617320 hasConceptScore W2081617320C86803240 @default.
• W2081617320 hasConceptScore W2081617320C89423630 @default.
• W2081617320 hasIssue "2" @default.
• W2081617320 hasLocation W20816173201 @default.
• W2081617320 hasLocation W20816173202 @default.
• W2081617320 hasOpenAccess W2081617320 @default.
• W2081617320 hasPrimaryLocation W20816173201 @default.
• W2081617320 hasRelatedWork W1581350964 @default.
• W2081617320 hasRelatedWork W1926346775 @default.
• W2081617320 hasRelatedWork W2000664484 @default.
• W2081617320 hasRelatedWork W2142253083 @default.
• W2081617320 hasRelatedWork W2240103830 @default.
• W2081617320 hasRelatedWork W2264800049 @default.
• W2081617320 hasRelatedWork W2352655741 @default.
• W2081617320 hasRelatedWork W2462005841 @default.
• W2081617320 hasRelatedWork W2610394211 @default.
• W2081617320 hasRelatedWork W4327657278 @default.
• W2081617320 hasVolume "160" @default.
• W2081617320 isParatext "false" @default.
• W2081617320 isRetracted "false" @default.
• W2081617320 magId "2081617320" @default.
• W2081617320 workType "article" @default.