Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081661572> ?p ?o ?g. }
- W2081661572 endingPage "36" @default.
- W2081661572 startingPage "25" @default.
- W2081661572 abstract "A quantitative assay based on real-time detection polymerase chain reaction (rtdPCR) was applied to analyze basal and metal-induced mRNA levels of two metallothionein (MT) isoforms (Cd–MT and Cu–MT) in organs of the terrestrial gastropod Helix pomatia. The results show that specific Cd–MT mRNA levels increase with Cd tissue burden, identifying hepatopancreas and gut as the main organs of Cd accumulation and, accordingly, the predominant organs of Cd-MT mRNA expression. In situ hybridization localized this isoform in epithelial cells of hepatopancreas, gut, and kidney. In contrast to the observed Cd-dependent inducibility of the Cd-binding MT isoform, gene expression of the Cu-binding MT could not be induced by either Cd or Cu exposure. Only very low mRNA amounts of the Cu–MT isoform were found in snail hepatopancreas and kidney, whereas the mantle exhibited high basal mRNA levels of this isoform. In situ localization revealed that the Cu-MT gene expression was restricted to one cell type, the so-called rhogocytes, which are present to various extents in the different organs examined. These results suggest a metal-specific sharing of functions between the two MT isoforms. The Cd–MT isoform apparently plays a crucial role in Cd detoxification, as demonstrated by the inducibility of this isoform, as well as its specific localization in the main metabolic and Cd storing organs. The predominant presence of Cu-MT in rhogocytes of snail mantle strengthens the hypothesis that this isoform may regulate Cu availability in hemocyanin synthesis." @default.
- W2081661572 created "2016-06-24" @default.
- W2081661572 creator A5007975306 @default.
- W2081661572 creator A5013042945 @default.
- W2081661572 creator A5018138430 @default.
- W2081661572 creator A5059030011 @default.
- W2081661572 creator A5067749952 @default.
- W2081661572 creator A5082653476 @default.
- W2081661572 creator A5086791556 @default.
- W2081661572 date "2003-07-01" @default.
- W2081661572 modified "2023-10-17" @default.
- W2081661572 title "Localization and quantification of Cd- and Cu-specific metallothionein isoform mRNA in cells and organs of the terrestrial gastropod Helix pomatia" @default.
- W2081661572 cites W140508311 @default.
- W2081661572 cites W1459042942 @default.
- W2081661572 cites W1495265866 @default.
- W2081661572 cites W1521070196 @default.
- W2081661572 cites W1535794217 @default.
- W2081661572 cites W1601228238 @default.
- W2081661572 cites W1604713610 @default.
- W2081661572 cites W1657501998 @default.
- W2081661572 cites W169117390 @default.
- W2081661572 cites W1769449718 @default.
- W2081661572 cites W1843446143 @default.
- W2081661572 cites W1878969453 @default.
- W2081661572 cites W196233321 @default.
- W2081661572 cites W1970538187 @default.
- W2081661572 cites W1970730404 @default.
- W2081661572 cites W1972067513 @default.
- W2081661572 cites W1981765089 @default.
- W2081661572 cites W1985329347 @default.
- W2081661572 cites W1985779311 @default.
- W2081661572 cites W1989732261 @default.
- W2081661572 cites W1993281215 @default.
- W2081661572 cites W1994116440 @default.
- W2081661572 cites W1994951153 @default.
- W2081661572 cites W1996270671 @default.
- W2081661572 cites W2012090817 @default.
- W2081661572 cites W2012293139 @default.
- W2081661572 cites W2013527534 @default.
- W2081661572 cites W2013697242 @default.
- W2081661572 cites W2017770464 @default.
- W2081661572 cites W2024167657 @default.
- W2081661572 cites W2032795893 @default.
- W2081661572 cites W2033368804 @default.
- W2081661572 cites W2034057956 @default.
- W2081661572 cites W2037968874 @default.
- W2081661572 cites W2043030760 @default.
- W2081661572 cites W2044161215 @default.
- W2081661572 cites W2051626724 @default.
- W2081661572 cites W2058953608 @default.
- W2081661572 cites W2060852569 @default.
- W2081661572 cites W2060913542 @default.
- W2081661572 cites W2060999771 @default.
- W2081661572 cites W2079490466 @default.
- W2081661572 cites W2094741111 @default.
- W2081661572 cites W2096095895 @default.
- W2081661572 cites W2097195192 @default.
- W2081661572 cites W2103544286 @default.
- W2081661572 cites W2106769439 @default.
- W2081661572 cites W2130385250 @default.
- W2081661572 cites W2141109284 @default.
- W2081661572 cites W2141158518 @default.
- W2081661572 cites W2151093371 @default.
- W2081661572 cites W2157142316 @default.
- W2081661572 cites W2184761538 @default.
- W2081661572 cites W2344392841 @default.
- W2081661572 cites W2413914254 @default.
- W2081661572 cites W2417206285 @default.
- W2081661572 cites W2479413460 @default.
- W2081661572 cites W2496067894 @default.
- W2081661572 cites W61127452 @default.
- W2081661572 doi "https://doi.org/10.1016/s0041-008x(03)00148-0" @default.
- W2081661572 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12831780" @default.
- W2081661572 hasPublicationYear "2003" @default.
- W2081661572 type Work @default.
- W2081661572 sameAs 2081661572 @default.
- W2081661572 citedByCount "72" @default.
- W2081661572 countsByYear W20816615722012 @default.
- W2081661572 countsByYear W20816615722013 @default.
- W2081661572 countsByYear W20816615722014 @default.
- W2081661572 countsByYear W20816615722015 @default.
- W2081661572 countsByYear W20816615722016 @default.
- W2081661572 countsByYear W20816615722017 @default.
- W2081661572 countsByYear W20816615722018 @default.
- W2081661572 countsByYear W20816615722019 @default.
- W2081661572 countsByYear W20816615722020 @default.
- W2081661572 countsByYear W20816615722021 @default.
- W2081661572 crossrefType "journal-article" @default.
- W2081661572 hasAuthorship W2081661572A5007975306 @default.
- W2081661572 hasAuthorship W2081661572A5013042945 @default.
- W2081661572 hasAuthorship W2081661572A5018138430 @default.
- W2081661572 hasAuthorship W2081661572A5059030011 @default.
- W2081661572 hasAuthorship W2081661572A5067749952 @default.
- W2081661572 hasAuthorship W2081661572A5082653476 @default.
- W2081661572 hasAuthorship W2081661572A5086791556 @default.
- W2081661572 hasConcept C104317684 @default.
- W2081661572 hasConcept C105580179 @default.
- W2081661572 hasConcept C147483822 @default.