FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2081688632> ?p ?o ?g. }

• W2081688632 endingPage "6068" @default.
• W2081688632 startingPage "6063" @default.
• W2081688632 abstract "Trehalose is a nonreducing disaccharide and has a wide range of applications in food and biorelated industry. This sugar can be synthesized from maltose in one step by trehalose synthase. In this study, we attempted to overproduce trehalose synthase from Picrophilus torridus (PTTS), a thermoacidophilic archaea, in Escherichia coli. However, overproduction of PTTS was hampered when the T7 promoter-driven PTTS gene (PT7-PTTS) on a multicopy plasmid was employed in E. coli. The factors limiting PTTS production were identified in a systematic way, including the codon bias, plasmid instability, a redundant gene copy, a high basal level of PTTS, and metabolic burden resulting from the mutlicopy plasmid DNA and antibiotics. To overcome these difficulties, an E. coli strain was developed with insertion of PT7-PTTS into the chromosome and enhanced expression of genomic argU tRNA and ileX tRNA genes. Without the selective pressure, the constructed producer strain was able to produce a stable and high-level production of recombinant PTTS. Overall, we proposed a simple and effective method to address the issue that is most commonly raised in overproduction of heterologous proteins by E. coli." @default.
• W2081688632 created "2016-06-24" @default.
• W2081688632 creator A5003403014 @default.
• W2081688632 creator A5008513694 @default.
• W2081688632 creator A5044839154 @default.
• W2081688632 creator A5049958886 @default.
• W2081688632 creator A5063327912 @default.
• W2081688632 creator A5073452348 @default.
• W2081688632 creator A5091694841 @default.
• W2081688632 date "2012-06-01" @default.
• W2081688632 modified "2023-09-26" @default.
• W2081688632 title "Strategy for Stable and High-Level Expression of Recombinant Trehalose Synthase in Escherichia coli" @default.
• W2081688632 cites W18352336 @default.
• W2081688632 cites W1971094080 @default.
• W2081688632 cites W1975993749 @default.
• W2081688632 cites W1978440870 @default.
• W2081688632 cites W1979452721 @default.
• W2081688632 cites W1983625008 @default.
• W2081688632 cites W1988314246 @default.
• W2081688632 cites W2018977702 @default.
• W2081688632 cites W2033229501 @default.
• W2081688632 cites W2036026814 @default.
• W2081688632 cites W2037760587 @default.
• W2081688632 cites W2052367587 @default.
• W2081688632 cites W2055831142 @default.
• W2081688632 cites W2057470797 @default.
• W2081688632 cites W2060818115 @default.
• W2081688632 cites W2068518785 @default.
• W2081688632 cites W2070639803 @default.
• W2081688632 cites W2073101425 @default.
• W2081688632 cites W2082042773 @default.
• W2081688632 cites W2088169918 @default.
• W2081688632 cites W2101533498 @default.
• W2081688632 cites W2108605550 @default.
• W2081688632 cites W2114967847 @default.
• W2081688632 cites W2116137883 @default.
• W2081688632 cites W2156350829 @default.
• W2081688632 cites W2161420671 @default.
• W2081688632 cites W2321930117 @default.
• W2081688632 cites W4254321567 @default.
• W2081688632 doi "https://doi.org/10.1021/jf301593e" @default.
• W2081688632 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22612301" @default.
• W2081688632 hasPublicationYear "2012" @default.
• W2081688632 type Work @default.
• W2081688632 sameAs 2081688632 @default.
• W2081688632 citedByCount "15" @default.
• W2081688632 countsByYear W20816886322015 @default.
• W2081688632 countsByYear W20816886322017 @default.
• W2081688632 countsByYear W20816886322018 @default.
• W2081688632 countsByYear W20816886322019 @default.
• W2081688632 countsByYear W20816886322020 @default.
• W2081688632 countsByYear W20816886322021 @default.
• W2081688632 countsByYear W20816886322022 @default.
• W2081688632 countsByYear W20816886322023 @default.
• W2081688632 crossrefType "journal-article" @default.
• W2081688632 hasAuthorship W2081688632A5003403014 @default.
• W2081688632 hasAuthorship W2081688632A5008513694 @default.
• W2081688632 hasAuthorship W2081688632A5044839154 @default.
• W2081688632 hasAuthorship W2081688632A5049958886 @default.
• W2081688632 hasAuthorship W2081688632A5063327912 @default.
• W2081688632 hasAuthorship W2081688632A5073452348 @default.
• W2081688632 hasAuthorship W2081688632A5091694841 @default.
• W2081688632 hasConcept C104317684 @default.
• W2081688632 hasConcept C108305142 @default.
• W2081688632 hasConcept C112243037 @default.
• W2081688632 hasConcept C124420848 @default.
• W2081688632 hasConcept C22744801 @default.
• W2081688632 hasConcept C2778452849 @default.
• W2081688632 hasConcept C2778910516 @default.
• W2081688632 hasConcept C40767141 @default.
• W2081688632 hasConcept C547475151 @default.
• W2081688632 hasConcept C55493867 @default.
• W2081688632 hasConcept C6350086 @default.
• W2081688632 hasConcept C86803240 @default.
• W2081688632 hasConcept C89423630 @default.
• W2081688632 hasConceptScore W2081688632C104317684 @default.
• W2081688632 hasConceptScore W2081688632C108305142 @default.
• W2081688632 hasConceptScore W2081688632C112243037 @default.
• W2081688632 hasConceptScore W2081688632C124420848 @default.
• W2081688632 hasConceptScore W2081688632C22744801 @default.
• W2081688632 hasConceptScore W2081688632C2778452849 @default.
• W2081688632 hasConceptScore W2081688632C2778910516 @default.
• W2081688632 hasConceptScore W2081688632C40767141 @default.
• W2081688632 hasConceptScore W2081688632C547475151 @default.
• W2081688632 hasConceptScore W2081688632C55493867 @default.
• W2081688632 hasConceptScore W2081688632C6350086 @default.
• W2081688632 hasConceptScore W2081688632C86803240 @default.
• W2081688632 hasConceptScore W2081688632C89423630 @default.
• W2081688632 hasIssue "23" @default.
• W2081688632 hasLocation W20816886321 @default.
• W2081688632 hasLocation W20816886322 @default.
• W2081688632 hasOpenAccess W2081688632 @default.
• W2081688632 hasPrimaryLocation W20816886321 @default.
• W2081688632 hasRelatedWork W1985426194 @default.
• W2081688632 hasRelatedWork W2030235571 @default.
• W2081688632 hasRelatedWork W2038624513 @default.
• W2081688632 hasRelatedWork W2041543427 @default.
• W2081688632 hasRelatedWork W2062613044 @default.

• next